南方红豆杉水提物联合顺铂抑制人肺癌A549细胞及对耐药基因的影响  

Study on inhibitory action of proliferation of human lung carcinoma A549 cells with aqueous extract of Taxus ehinensis, var combining Cisplatin and the effects on resistance genes

作　　者：徐金田[1] 李萍[1] 毛佳斌[1] 郑晟超 

机构地区：[1]杭州师范大学附属医院结核科,杭州310015

出　　处：《中国医师杂志》2016年第12期1790-1793,共4页Journal of Chinese Physician

基　　金：浙江省自然基金项目（Y2110983）

摘　　要：目的研究南方红豆杉水提物联合顺铂（DDP）对肺癌A549细胞增殖的抑制作用和对耐药基因的影响。方法将A549细胞分为不同浓度南方红豆杉水提物组及空白组，用CCK-8法检测药物作用48h后对细胞存活率的影响。根据上述结果，再将A549细胞分为DDP联合不同浓度南方红豆杉水提物组、DDP组、空白对照组，用CCK-8法检测药物作用48h后对细胞存活率的影响，用RT—PCR检测细胞内三磷酸腺苷（ATP）结合蛋白亚科B运载体1（ABCB1）、ATP结合蛋白亚科G运载体2（ABCG2）、ATP结合蛋白亚科C运载体1（ABCC1）基因表达变化。结果DDP12μg／ml组、DDP＋红豆杉400μg/ml组．DDP＋红豆杉800彬mI组、DDP＋红豆杉1200μg／ml组、DDP＋红豆杉1600μg/ml组对A549细胞的抑制率分别为44．36％、69．61％、74．73％、80．10％、80．63％；不同浓度红豆杉联合DDP引起耐药相关基因ABCC1、ABCB1的水平逐渐降低，呈剂量相关性，耐药相关基因ABCG2的表达无明显变化。结论南方红豆杉水提物联合DDP能抑制A549细胞生长，并能调节耐药基因ABCC1、ABCB1的表达。Objective To investigate the inhibitory effect of aqueous extract of Taxus chinensis. var （AETC） combining Cisplatin （DDP） on vitro cultured human lung carcinoma A549 cells, and the effects on resistance genes. Methods The A549 cells were divided into different concentrations of DDP groups, different concentrations of AETC groups, and blank group, and drug effect of 48 h with the method of cell counting kit-8 （CCK-8） and the effect on cell survival were detected. Based on the above results, then A549 cells were divided into DDP combining different concentrations of AETC groups, DDP group, blank control group, and drug effect of 48 h with the method of CCK-8 and the effect on cells survival were detected. The gene expressions of adenosine triphosphate （ATP）-binding cassette subfamily B member 1 trans-porter （ABCB1）, ABCG2, and ABCC1 were examined by polymerase chain reaction （RT-PCR）. Results Cisplatin 12 μg/ml （ DDP）, DDP ＋ ATEC 400 μg/ml, DDP ＋ ATEC 800 μg/ml, DDP ＋ ATEC 1 200 μg/ml, DDP ＋ ATEC 1 600 μg/ml, A549 cell inhibition rate of each group was 44. 36%, 69. 61%, 74.73%, 80. 10%, and 74. 73%, respectively; Different concentrations of AETC combining DDP could decrease the resistance related gene ABCC1, ABCB1 expressions, and correlated to the dose. AETC combi- ning DDP showed no effects on ABCG2 gene expression. Conclusions AETC combining DDP could inhibit the growth of A549 cells, and decrease the resistance-related gene ABCC1, ABCB1 expressions.

关 键 词：红豆杉属 顺铂/投药和剂量 肺肿瘤/中西医结合疗法/代谢 细胞增殖/药物作 用 ATP结合匣式转运子/代谢 

分 类 号：R734.2[医药卫生—肿瘤]