Caveolin-1在非酒精性脂肪性肝炎形成中的作用  

Studies on the role of Caveolin-I in non-alcoholic steatohepatitis

作　　者：李访贤 许国强[2] 陆静静 彭栋柱 马海芬 林冠斌 李燕 余海平 

机构地区：[1]宁波市北仑区人民医院消化内科,宁波315800 [2]浙江大学第一附属医院消化内科,杭州310000

出　　处：《中国医师杂志》2016年第12期1805-1807,1811,共4页Journal of Chinese Physician

基　　金：浙江省医药卫生一般研究计划（2012KYB187）

摘　　要：目的研究Caveolin-1在非酒精性脂肪性肝炎（NASH）发病中的作用。方法对C57BL／6小鼠予高脂肪高胆固醇饮食建立NASH的动物模型，利用实时荧光定量PCR和Westernblot法检测小鼠肝组织中Caveolin-1基因和蛋白表达的改变。结果实验组小鼠在高脂肪高胆固醇饮食16周后全部发生非酒精性脂肪性肝病（NAFLD），与对照组比较，NASH组小鼠肝脏Caveolin-1的mRNA和蛋白水平均显著增高。结论Caveolin-1在高脂肪高胆固醇饮食性NASH小鼠的肝组织中表达增加，可能与NASH的发病机制相关。Objective To explore the role of Caveolin-1 in the progression of non-alcoholic steato- hepatitis （NASH）. Methods C57BL/6 mouse model with NASH was established through feeding high fat and high cholesterol diet. Variation of expressed Caveolin-1 gene and protein in liver tissues of mice was de- tected with real-time fluorescence quantitative polymerase chain reaction （PCR） and Western blotting assay. Results The mice in the experimental group fed with high fat high cholesterol diet all developed non- alcoholic fatty liver disease （NAFLD） after 16 weeks, compared to the control group, the level of Caveolin- 1 mRNA and protein in the liver of mice with NASH were significantly enhanced. Conclusions The en- hancement of Caveolin-1 in liver of mice fed with high fat and high cholesterol diet may correlate with the occurrence of NASH.

关 键 词：窖蛋白1/代谢 脂肪肝/代谢 

分 类 号：R575.1[医药卫生—消化系统]