滴鼻大肠杆菌对高脂诱导肥胖小鼠糖脂代谢的影响  

Effects of intranasal Escherichia coli on glucolipid metabolism in high-fat diet-induced obese mice

作　　者：王正义[1] 甘霖莉 贾义平 崔恒敏[1] 邓俊良[1] 左之才[1] 

机构地区：[1]四川农业大学动物医学院,四川温江611130

出　　处：《中国病理生理杂志》2017年第1期60-66,共7页Chinese Journal of Pathophysiology

基　　金：四川省科技厅支撑项目(No.2013NZ0032)

摘　　要：目的:探讨滴鼻大肠杆菌对高脂诱导肥胖小鼠糖脂代谢的影响。方法:选取48只高脂诱导的肥胖小鼠作为肥胖组,48只非肥胖小鼠作为对照组,滴鼻40μL含4×10~9 CFUs大肠杆菌菌液,于感染前及感染后1、2、3、4 d检测2组小鼠血清糖脂代谢相关指标及肝脏脂质沉积情况。结果:与感染前比较,肥胖小鼠体质量、附睾脂肪组织质量及指数在感染后1~4d显著降低(P<0.05);血清空腹血糖(FBG)、空腹胰岛素(FINS)水平以及胰岛素抵抗指数(HOMA-IR)在2~4d显著升高(P<0.05);血清游离脂肪酸(FFA)、甘油三酯(TG)和极低密度脂蛋白(VLDL)水平在1~4 d显著升高总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDLC)水平在1~3d显著降低(P<0.05);肝脏质量、指数及TG含量在1~4d显著升高(P<0.05),肝细胞脂滴蓄积在感染后2和4d明显增多。与对照组比较肥胖组小鼠血清TC、LDL-C和HDL-C水平在感染后显著降低,其余指标均明显升高(P<0.05)。结论:滴鼻大肠杆菌可加剧肥胖小鼠糖脂代谢紊乱程度,促进其胰岛素抵抗及肝脏脂肪化的发生、发展。AIM：To study whether the pulmonary infection of Escherichia coli（E.coli） interferes the glucolipid metabolism in high-fat diet-induced obese mice.METHODS：High-fat diet-induced obese mice（n = 48） and normal chow-fed control mice（n = 48） were intranasally infused with 40μL fluid containing 4 × 10~9 CFUs E.coli.The serum,periepididymal adipose tissue and liver were obtained at 0 d,1 d,2d,3d and 4 d after infection.The body mass,periepididymal adipose tissue and liver were weighed,and the levels of fasting blood glucose（FBG）,fasting blood insulin（FINS）,free fatty acid（FFA） and very-low-density lipoprotein（VLDL） were measured by ELISA.The serum total cholesterol（TC）,triglycerides（TG）,high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C）,and hepatic TG contents were detected,and the hepatic steatosis was observed under microscope with oil red O staining.RESULTS：Compared with day 0,the body mass,fat mass and fat index were decreased significantly from day 1to day 4 after infection（P 〈 0.05）.The levels of FBG,FINS and HOMA-IR were apparently raised from day 2 to day 4 after infection（P 〈0.05）.The contents of serum FFA,TG and VLDL were increased markedly from day 1 to day 4 after infection（P 〈0.05）.However,the concentrations of serum TC,LDL-C and HDL-C were decreased obviously from day 1 to day 3（P 〈 0.05）.The liver mass,liver index and TG content were significantly increased from day 1 to day 4（P 〈0.05）.Consistently,the lipid droplet accumulation in the liver cells was increased obviously at day 2 and day 4 after infection.Compared with control group,except the levels of serum TC,LDL-C and HDL-C in obese group substantially decreased,the other indexes were increased by different degrees during the whole experiment period（P 〈 0.05）.CONCLUSION：Pulmonary infection of Escherichia coli exacerbates the disorder of glucose and lipid metabolism in high-fat diet-induced obese mice,which contributes the de

关 键 词：肥胖 感染 糖脂代谢 

分 类 号：R363[医药卫生—病理学]