高危型人乳头瘤病毒感染与microRNA-155在宫颈鳞状细胞癌组织和血清中的表达及意义  

Expressions and significances of high-risk human papillomavirus infection and microRNA-155 in cervical squamous cell cancinoma

作　　者：陈瑞萍[1] 麦振声[2] 徐建平[1] 

机构地区：[1]暨南大学附属第一医院妇产科,广州5106302 [2]佛山市第一人民医院妇产科,528000

出　　处：《妇产与遗传（电子版）》2016年第2期26-30,共5页Obstetrics-Gynecology and Genetics (Electronic Edition)

摘　　要：目的 探讨高危型人乳头瘤病毒（highrisk human papillomavirus,HR-HPV）感染与微小RNA-155在宫颈鳞状细胞癌组织及血清中的表达差异及临床意义. 方法 （1）选取宫颈鳞状细胞癌患者37例为研究组,另选取20例子宫肌瘤患者为对照组,收集两组病例的宫颈脱落细胞,宫颈组织以及血清标本. （2）采用导流杂交法检测人乳头瘤病毒（human papilloma virus,HPV）的基因型以及用荧光定量逆转录一聚合酶链反应（RT-qPCR）测定宫颈鳞状细胞癌组织及血清中微小RNA-155的表达. 结果 （1）研究组HR-HPV感染率明显高于对照组（x2=19.64,P＜0.01）;（2）研究组其组织及血清中的微小RNA-155表达均高于对照组（Z=-2.20,P＜0.05;Z=-4.75;P＜0.05）;（3） HR-HPV阳性的宫颈组织中微小RNA-155的表达高于HR-HPV阴性的宫颈组织（Z=-2.26,P＜0.05）. 结论 宫颈鳞状细胞癌患者宫颈组织与血清中miRNA-155的表达变化可能与HR-HPV感染有关.Objective To study the expressions of and significances of high-risk human papillomavirus infection and microRNA-155 in cervical squamous cell cancinoma. Methods Cervical tissues of 37 subjects with cervical squamous cell carcinoma obtained from hysterectomy or cervical conization were included in this study group. Another 20 cases from normal subjects served as controls. All the cases were collected the specimens from the cervical canal, cervical tissue and serum. Use the diversion hybrid method to detect HPV genotypes and fluorescence quantitative reverse transcription- polymerase chain reaction （ RT-qPCR） to detect miRNA-155 expression in the cervical tissues and serum. Results The infection rate of HR-HPV in study group was obviously higher than that of the controls χ2= 19. 64, P 〈0 . 01） . Both of the expression of miRNA-155 in the cervical lesions and the serum were higher in the group of cervical squamous cell carcinoma than those in the controls （Z= -2 . 20, P 〈 0. 05; Z = - 4. 15, P 〈 0. 0 5 ） . 3, The expression of miRNA-155 in HR-HPV positive cervical tissues is higher than that of HR-HPV negative cervical tissues （Z = -2 . 26, P 〈0. 05）. Conclusions The positive expression of miRNA-155 both in cervical squamous cell carcinoma lesions and serum were related to the infection of HR-HPV.

关 键 词：肿瘤 鳞状细胞 高危型人类乳头病毒 miRNA-155 

分 类 号：R737.33[医药卫生—肿瘤]