液相色谱三重四级杆质谱联用法同时测定豆芽中2,4-滴和6-苄基腺嘌呤  

Simultaneous Determination of 2,4-Dichlorophenoxyacetic and 6-Benzyladenine in Bean Sprouts by LC-MS/MS

作　　者：赵丽君 冉艳 安莎 杨昌彪 李占彬 

机构地区：[1]贵州省机械工业学校,贵阳550002 [2]贵州省分析测试研究院,贵阳550002

出　　处：《湖北农业科学》2016年第23期6228-6230,共3页Hubei Agricultural Sciences

基　　金：贵州省林业科研技术基金项目(黔林科合J字[2012]18号);国家国际科技合作专项(2011DFB41640)

摘　　要：建立一种超声提取-液相色谱三重四级杆联用法（LC-MS/MS）同时测定豆芽中2,4-滴、6-苄基腺嘌呤2种植物生长调节剂的方法。酸化甲醇作为提取溶剂,涡旋2 min,超声波提取10 min,酸化甲醇定容;反相色谱柱分离,电喷雾离子化（ESI＋/-）和多离子反应监测（MRM）方式定量测定;加标回收试验2,4-滴添加浓度分别为5,10,20μg/kg,6-苄基腺嘌呤添加浓度为5,10,20μg/kg,平均回收率为85.0%-105.3%,相对标准偏差（RSD）1.0%-3.2%;2,4-滴,6-苄基腺嘌呤检出限分别为1.0、2.0μg/kg。方法快捷、简便、样品前处理时间少,适合食品监管中大批量样品快速测定使用。A method for the simultaneous determination of 2,4-Dichlorophenoxyacetic and 6-benzyladenine in bean sprouts was developed by ultrasonic extraction and LC-MS / MS. Samples were extracted using acidulated methanol as extraction solvent,with 2 min vortex,10 min ultrasonic extraction, and constant volume by acidulated methanol,then were separated with reversed phase column and determined quantitatively with the multiple reaction monitoring（MRM） mode and electrospray ionization（ESI＋/-）. The standard recovery tests showed that the additive concentration of 2,4-Dichlorophenoxyacetic were 5,10,20μg / kg respectively, the additive concentration of 6-benzyladenine were 5,10,20 μg / kg respectively,and the average recovery was in the range of 85.0%~105.3%, the relative standard deviations（RSDs） was in 1.0%~3.2%,and the limit of detections of2,4-Dichlorophenoxyacetic and 6-benzyladenine was 1 μg / kg and 2 μg / kg respectively. The method was rapid, convenient and cut down pretreatment time of samples, suitable for the rapid determination of large batch samples.

关 键 词：2 4-滴 6-苄基腺嘌呤 液相色谱三重四级杆质谱联用法(LC-MS/MS) 豆芽 

分 类 号：S481.2[农业科学—农药学] TS202.3[农业科学—植物保护]