小球藻类金属硫蛋白抗紫外辐射的作用研究  

Protective effect of Zn- MT- like extracted from C. vulgaris for NIH /3T3 cells against ultraviolet irradiation

作　　者：杨红[1,2] 黄晓淳[1,3] 黄婧筠 彭爱红[1] 黄志勇[1] 

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]龙岩学院科研处,福建龙岩364012 [3]厦门华厦学院健康管理系,福建厦门361021

出　　处：《日用化学工业》2017年第1期28-31,共4页China Surfactant Detergent & Cosmetics

基　　金：厦门市海洋经济发展专项(14GZP67NF31);集美大学创新团队基金资助项目(2010A007)

摘　　要：建立了小鼠胚胎成纤维(NIH/3T3)细胞紫外辐射损伤的实验模型,考察小球藻类金属硫蛋白(Zn-MT-like)对NIH/3T3细胞紫外辐射的保护作用。结果表明,小球藻Zn-MT-like对NIH/3T3细胞的生长无抑制作用,且具有较强的抗紫外辐射能力。质量浓度为10 g/L的小球藻Zn-MT-like可显著减少NIH/3T3细胞空泡、降低细胞裂解的程度,可使经紫外辐射的NIH/3T3细胞保持较好的活性。A laboratory model for testing ultraviolet irradiation damage of fibroblast （ NIH/3T3 ） of mouse embryo was established ; and it was applied for examination of protective effect of Zine- binding metallothionein - like （Zn- MT- like） extracted from C. vulgaris for NIH/3T3 cells against ultraviolet irradiation. Results showed that the Zn - MT - like presents a strong protective effect for the NH/3T3 cells from irradiation damage. For example ,the Zn - MT - like at mass concentration of 10 g/L can effectively reduce the NIH/3T3 cell cavity and cell fragmentation under the ultraviolet irradiation, and maintaining rather good cell activity.

关 键 词：抗紫外辐射 小球藻 类金属硫蛋白 NIH/3T3细胞模型 

分 类 号：R151[医药卫生—营养与食品卫生学]