TRPV5/6通道在急性髓系白血病中的表达研究  

Expression of transient receptor potential channel 5 and 6 in acute myeloid leukemia

作　　者：吴超[1] 杨新宇[1] 李璇[1] 周明[1] 肖佩玲[1] 

机构地区：[1]湖南省人民医院血液科,湖南长沙410005

出　　处：《中国现代医学杂志》2017年第1期71-76,共6页China Journal of Modern Medicine

摘　　要：目的探讨瞬时受体电位通道V5(TRPV5)、瞬时受体电位通道V6(TRPV6)在急性髓系白血病(AML)中的表达。方法提取AML患者骨髓细胞、捐献者造血干细胞,以及AML细胞株的蛋白和总RNA,运用实时荧光定量聚合酶链反应(q RT-PCR)及Western blot检测TRPV5/6基因和蛋白的表达水平,并分析AML患者临床指标与TRPV5/6表达水平的相关性。结果初治组、复发组、细胞株组结果显示,TRPV5/6的表达高于对照组,差异有统计学意义(P<0.05),而完全缓解组与对照组TRPV5/6的表达比较,差异无统计学意义(P>0.05)。AML患者TRPV5/6表达与骨髓原始细胞百分比呈正相关(P<0.05),而与外周血白细胞计数、血红蛋白、血小板计数、性别、年龄及骨髓染色体核型比较,差异无统计学意义(P>0.05)。结论 TRPV5/6通道在AML中表达上调,且与肿瘤负荷相关,有望成为白血病诊断的新型标志物,以及预后评估的分子靶点。Objective To observe the expressions of transient receptor potential channel TRPV 5 and TRPV6 in acute myeloid leukemia （AML）. Methods The protein and total RNA were extracted from bone marrow ceils of AML patients, hematopoietic stem ceils of healthy donors and AML cell lines. Quantitative real-time polymerase chain reaction and Western blot were used respectively to examine the expression levels of TRPV5 and TRPV 6. The relationships between TRPV 5 and TRPV6 expressions and clinical features of the patients were analyzed. Results The expressions of TRPV5 and TRPV6 in the initial treatment group, the relapse group and the cell line group were significantly higher than that in the control group （P〈 0.05）. The expressions of TRPV5 and TRPV6 in the complete remission group showed no significant difference from that in the control group （P 〉 0.05）. The expressions of TRPV5 and TRPV6 in the AML patients had positive correlations with the percentage of bone marrow progenitor cells （P 〈 0.05）, but had no obvious correlation with WBC count, hemoglobin, platelet count of peripheral blood, sex, age, or bone marrow chromosome （P 〉 0.05）. Conclusions TRPV5 and TRPV6 are excessively up-regulated in acute myeloid leukemia and correlated with tumor load of the patients. They could be new markers of prognosis and molecular targets for diagnosis of AML.

关 键 词：急性髓系白血病 TRPV5/6通道 骨髓细胞 

分 类 号：R733[医药卫生—肿瘤]