pAdKDR/CD/TK双自杀基因系统对肝癌细胞的杀伤作用的试验研究  

The study on killing effect of KDR promoter driving double suicide gene on human hepatic cancer HepG2 cells

作　　者：谷洋[1] 刘志毅[1] 金虎[1] 刘明[1] 孙铁梁[1] 

机构地区：[1]吉林大学第四医院普通外科,吉林长春130011

出　　处：《中国实验诊断学》2017年第1期132-135,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅自然科学基金面上项目(201115117)

摘　　要：目的探讨含KDR启动子的双自杀基因CD/TK对肝癌HepG2细胞的杀伤作用。方法观察不同前药对HepG2细胞、血管内皮细胞ECV304和LST174细胞的杀伤作用,评估KDR启动子的靶向杀伤作用。观察不同前药对转染不同自杀基因的HepG2细胞的杀伤作用,及旁观者效应。结果转染腺病毒的HepG2细胞及血管内皮ECV304细胞对前药具有较高的敏感性,而感染腺病毒的LST174细胞对前药不敏感(P<0.05)。当转染细胞混合比例为50%时,应用前药后,转染CD/TK,CD,TK的HepG2细胞生存率分别为11.8±1.2%、41.3±3.7%、43.1±2.3%(P<0.05)。结论含KDR启动子的双自杀基因CD/TK对肝癌HepG2细胞具有高度靶向杀伤作用;联合用药对转染双自杀基因的肝癌HepG2细胞具有协调增强杀伤作用。Objective To investigate the killing effect of KDR promoter driving double suicide gene on human hepatic cancer HepG2 cells.Methods To observe the killing effect of different kind of pre-drug on HepG2 cells,ECV304 cells and LS174 Tcells,and evaluated the targeted killing effect of KDR.To observe the killing effect and standby effect of different kind of pre-drug on HepG2 cells.Results The HepG2 cells and ECV304 cells transfected with recombined adenovirus had high sensitivity to pre-drug.And the LS17T4 cells transfected with recombined adenovirus had no sensitivity to pre-drug（P〈0.05）.The HepG2 cells which transfected CD/TK,CD and TK genes,the survival rate of are 11.8±1.2%、41.3±3.7%、43.1±2.3% respectively（P〈0.05）after using the pre-drug.Conclusion KDR promoter driving double suicide gene had high targed killing effect on human hepatic cancer HepG2 cells.There were enhanced killing effect on transfected double suicide genes of human hepatic cancer HepG2 cells when used combined pre-drugs.

关 键 词：双自杀基因 KDR启动子 肝癌细胞 基因 

分 类 号：R735.7[医药卫生—肿瘤]