脑信号蛋白6D和激酶插入区受体2在大肠腺癌组织的表达及其临床意义  

Expression of brain signal protein 6D and kinase domain receptor 2 in colorectal glandular cancer and their significance

作　　者：朱瑞平[1] 董晓欢[1] 张林[1] 潘雪凯 王小康[2] 张弛[2] 王诗培[2] 冯茂辉[2] 谢伟[2] 

机构地区：[1]武汉大学中南医院消化内科,430071 [2]武汉大学肿瘤外科肿瘤生物学行为湖北省重点实验室武汉市腹膜癌临床医学研究中心,430071

出　　处：《中华实验外科杂志》2017年第1期48-51,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81072152）;湖北省自然科学基金（2015CFA027）;湖北省卫生计生委科研基金（WJ2015MA010）;武汉市腹膜癌临床医学研究中心资助项目（2015060911020462）

摘　　要：目的探讨脑信号蛋白6D（Sema6D）和激酶插入区受体2（KDR）在大肠腺癌中的表达及其与大肠腺癌发生发展的关系。方法利用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法和反转录-聚合酶链反应（RT-PCR）方法检测Sema6D和KDR蛋白和mRNA在大肠腺癌和癌旁正常组织中的表达及其与临床病理之间的关系，分析两者之间的相关性。结果Sema6D和KDR在大肠腺癌中的表达明显高于正常组织（70.3%比45.3%，P＝0.004、76.6%比48.4%，P＝0.002），且与大肠腺癌淋巴结转移、Duke’s分期、分化程度呈正相关，两者在大肠腺癌中的表达具有正相关。结论Sema6D、KDR可能在大肠腺癌的发生发展中具有重要作用。Objective To investigate the expression and clinicopathologic significance of brain sig- nal protein 6D （Sema6D） and kinase domain receptor 2 （KDR） in human colorectal glandular cancer, and discuss the relationship between them in the occurrence and development of human colorectal glandular canc- er. Methods Immunohistochemistry and reverse transcriptase -polymerase chain reaction （RT- PCR） were performed to assay the expression and clinicopathologic significance of Sema6D and KDR in the colorectal glandular cancer. The relationship between Sema6D and KDR was also analyzed. Results The expression of Sema6D and KDR was striking higher in the eolorectal glandular cancer （ 70. 3% vs. 45.3% , P = 0. 004,76. 6% vs. 48.4% ,P =0. 002）, and the expression of Sema6D and KDR was positively correlated with the differentiation, lymph node metastasis and Duke＇ s staging, while they had no correlation with other clinical factors including age, gender and so on. The expression of Sema6D was positively correlated with KDR. Conclusion Sema6D and KDR play the important role in the occurrence and development of the colorectal glandular cancer.

关 键 词：结直肠腺癌 脑信号蛋白6D 激酶插入区受体2 

分 类 号：R735.34[医药卫生—肿瘤]