Tat结合蛋白30在结直肠腺瘤及结直肠癌组织中的表达  

The expression of Tat interacting protein 30 in colorectal adenoma and colorectal carcinoma

作　　者：樊丽伟[1] 李洵[1] 孟晓明[1] 宋鑫[1] 汪景坤 付晓霞[1] 王爱民[1] 

机构地区：[1]解放军252医院消化内科,河北保定071000

出　　处：《中华实验外科杂志》2017年第1期52-54,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测结直肠癌、结直肠腺瘤及正常结直肠黏膜中Tat结合蛋白30（TIP30）基因的表达，探讨结直肠腺瘤恶变过程中TIP30的作用及价值。方法收集结直肠癌组织60例，结直肠腺瘤组织80例，正常结直肠黏膜组织40例，采用免疫组织化学技术及荧光定量聚合酶链反应（PCR）方法检测TIP30的表达。结果免疫组织化学技术结果显示正常结直肠黏膜、结直肠腺瘤、结直肠癌中TIP30的阳性表达率分别为95.0%、67.5%、30.0%，差异有统计学意义（P＝0.000）。结直肠管状腺瘤、结直肠混合性腺瘤、结直肠绒毛状腺瘤中TIP30的阳性表达率分别为86.7%、66.7%、40.0%，差异有统计学意义（P＝0.003）。同时研究结果表明TIP30的表达与肿瘤的Duckes分期（P＝0.008）、分化程度（P＝0.015）、淋巴结转移（P＝0.024）明显相关，而与性别（P＝0.955）、年龄（P＝0.815）无明显相关。荧光定量PCR显示TIP30特异性扩增，其表达量在正常结直肠黏膜、结直肠管状腺瘤、结直肠混合性腺瘤、结直肠绒毛状腺瘤及结直肠癌组织中分别为25.135±1.474、18.701±1.596、12.412±1.677、6.579±0.795、2.137±0.535，F＝250.931，P＝0.015。结论TIP30可能对结直肠腺瘤、结直肠癌的发生与发展起到一定抑制作用。Objective The aim of this study is to detect the expression levels of Tat interacting protein 30 （TIP30） in colorectal carcinoma, colorectal adenomas, normal colorectal mucosa, and analyze the relationships among different histological types of colorectal adenomas and colorectal carcinoma.Methods We collected 60 colorectal carcinoma tissues, 40 normal colorectal mucosa tissues, 80 colorectal adenomas. Immunohistochemistry and fluorescence quantitative polymerase chain reaction （PCR） were used to detect the expression of TIP30 in the tissues.Results The results of the immunohistochemistry were that the positive expression rates of TIP30 in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues were 95.0%, 67.5%, 30.0%, respectively, with significant differences among them （P=0.000）. The positive expression rates of TIP30 in tubular adenoma, mixed pattern of adenoma, villous adenoma were 86.7%, 66.7%, 40.0%, respectively, also with significant differences among them （P=0.003）. There was a significant correlation between reduced expression of TIP30 and unfavorable variables, including nodal metastasis （P=0.024）, Dukes stage （P=0.008）, differentiation degree （P=0.015）. There was no obvious difference in age （P=0.815） and sex （P=0.955）. Fluorescence quantitative PCR showed characterized amplification about TIP30. The expression of TIP30 mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma were 25.135±1.474, 18.701±1.596, 12.412±1.677, 6.579±0.795, 2.137±0.535, respectively, F=250.931, P=0.015.Conclusion TIP30 may participate in the inhibition of the occurrence and development of colorectal adenoma and colorectal carcinoma.

关 键 词：结直肠腺瘤 结直肠癌 Tat结合蛋白30 免疫组织化学技术 荧光定量聚合酶链反应 

分 类 号：R735.34[医药卫生—肿瘤]