沉默CDC37抑制结肠癌细胞Lovo和Ls174T侵袭迁移  

CDC37 Silencing Inhibits the Invasion and Migration in Colon Cancer Lovo and Ls174T Cells

作　　者：朱剑军[1] 燕芳[2] 陶佳[3] 朱晓华[1] 刘家有[1] 邓世山[1] 

机构地区：[1]川北医学院基础医学院人体解剖学教研室,四川南充637100 [2]重庆大学生命科学学院,重庆400044 [3]川北医学院基础医学院病理教研室,四川南充637100

出　　处：《中国生物化学与分子生物学报》2017年第1期58-65,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.811724496);四川省教育厅基金项目(No.13ZB0242)资助~~

摘　　要：前期研究发现周期分裂蛋白37(cell division cycle 37,CDC37)的异常表达可能与结直肠腺癌的转移有关,但是其作用机制尚不清楚。本研究通过实时定量PCR和免疫印迹实检测CDC37在不同肠癌细胞中的m NRA和蛋白质表达水平,结果发现CDC37在结肠癌Lovo和Ls174T细胞中表达较高。siRNA靶向沉默CDC37表达后,细胞侧向迁移能力、垂直迁移能力、侵袭活性均显著降低。实时定量PCR和免疫印迹实验结果发现,敲除CDC37后,MMP9和MMP2的m NRA和蛋白质表达水平均显著下调。以上研究结果表明,CDC37通过调节MMP9和MMP2等侵袭转移关键分子的表达在结肠癌细胞侵袭转移过程中发挥关键作用。It has been shown that abnormal expression of CDC37（ cell division cycle 37） might be related with the metastasis of colorectal adenocarcinoma in our previous study. But it is unknown how CDC37 regulates the metastasis of colorectal adenocarcinoma. Herein,we found that the mRNA and protein expression of CDC37 in Lovo and Ls174 T cells were higher than that in the other colorectal adenocarcinoma cells through real-time PCR and Western Blot. In addition,our results showed that the lateral migration ability of Lovo and Ls174 T cells were decreased after silencing CDC37 via wound healing assay. Moreover,Transwell assay showed that the vertical migration ability of Lovo and Ls174 T cells was decreased after knocking-down CDC37. Also we found that the invasion activity of the Lovo and Ls174 T cells was reduced after knocking-down CDC37 via Transwell assay. Furthermore,the mRNA and protein expression of MMP9 and MMP2 were significantly decreased after silencing CDC37 in Lovo and Ls174 T cells. Together,these results suggest that CDC37 plays an important role in invasion and migration process through regulating MMP9 and MMP2 in the development of colorectal adenocarcinoma.

关 键 词：CDC37 结肠癌 肿瘤转移 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]