白细胞介素-35对肝癌细胞株HepG2增殖和迁移能力的影响  

Effects of interleukin-35 on proliferation and migration of HepG2 cell line

作　　者：王海明[1] 刘浩润[2] 肖漓[2] 赵洪强[2] 高钰[2] 崔洪涛[2] 李为民[2] 

机构地区：[1]河北北方学院研究生部,张家口075000 [2]解放军309医院肝胆外科

出　　处：《中华肝胆外科杂志》2017年第1期12-15,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨白细胞介素-35（IL-35）对肝癌细胞株HepG2增殖活性、迁移和侵袭能力的影响。方法采用酶联免疫吸附法（ELISA）法检测肝细胞癌（HCC）患者和正常体检者外周血IL-35含量；CCK-8法检测外源性IL-35在体外对肝癌细胞增殖活性的影响；Transwell侵袭和划痕实验检测外源性IL-35对肝癌细胞侵袭和迁移能力的影响。结果IL-35在HCC患者外周血的IL-35含量[（i18．45±28．66）ng／L]显著高于健康体检者外周血[（39．56±11．15）ng/L，P〈0．05]；加入外源性人重组IL-35可以显著促进HepG2细胞的增殖活性、侵袭和迁移能力（P〈0．05）。结论IL-35具有促进肝癌细胞增殖、迁移和侵袭的生物学特性。Objective To investigate effects of interleukin-35 （IL-35） on proliferation, invasion and migration of a HepG2 cell line in vitro. Methods Enzyme-linked immunosorbent assay （ELISA） was used to determine peripheral blood expression levels of IL-35 in hepatocellular carcinoma patients and in normal healthy subjects. The effects of exogenous IL-35 on cell proliferation activity of HepG2 in vitro were measured using CCK-8 assay. The transwell invasion and scratch test were used to study the invasive and migration abilities. Results The content of IL-35 protein in hepatocellular carcinoma patients （HCC） was sig- nificantly higher than in healthy individuals [ （ 118.45 ± 28.66） ng/L vs （39.56 ± 11.15 ） ng/L, P 〈 0.05 ]. Exogenous IL-35 significantly promoted proliferation, invasion and migration abilities of HepG2 cells in vitro （P 〈 0. 05 ）. Conclusions The level of IL-35 was elevated significantly in hepatocellular carcinoma patients. IL-35 had biological characteristics of promoting HCC cell proliferation, invasion and migration.

关 键 词：白细胞介素-35 肝细胞癌 增殖活性 侵袭 迁移 

分 类 号：R735.7[医药卫生—肿瘤]