采油微生物在多孔介质中的迁移滞留机制  

Migration and retention mechanism of microorganisms for oil recovery in porous media

作　　者：毕永强[1,2] 俞理[2,3] 修建龙[2,3] 伊丽娜[2,3] 黄立信[2,3] 王天源 Bi Yongqiang Yu Li Xiu Jianlong Yi Lina Huang Lixin Wang Tianyuan(Institute of Porous Flow ~ Fluid Mechanics, University of Chinese Academy of Sciences, Hebei Langf ang 065007, China Langf ang Branch, PetroChina Research Institute of Petroleum Exploration and Development, Hebei Langf ang 065007, China CNPC Key Laboratory of Enhanced Oil Recovery, Hebei Lang fang 065007, China)

机构地区：[1]中国科学院大学渗流流体力学研究所,河北廊坊065007 [2]中国石油勘探开发研究院廊坊分院,河北廊坊065007 [3]中国石油天然气集团公司三次采油重点实验室,河北廊坊065007

出　　处：《石油学报》2017年第1期91-98,共8页Acta Petrolei Sinica

基　　金：国家高技术研究发展计划(863)项目(2013AA064402);中国石油天然气集团公司重大科技专项"华北油田上产稳产800万吨关键技术研究与应用"(2014E-3507)资助

摘　　要：采油微生物在多孔介质迁移过程中的滞留特征明显。以2株典型采油微生物Pseudomonas aeruginosa WJ-1和Bacillus subtilis SLY-3为研究对象,通过等温吸附实验和流动实验研究了其迁移滞留规律。研究结果表明:多孔介质的比表面积越大,吸附位点越多,菌体的吸附量越大。菌体和多孔介质表面的疏水性关系影响菌体在多孔介质表面的吸附量。采油微生物在油藏多孔介质环境中的总菌浓度一般低于3.5×108cfu/mL,在此条件下,采油微生物在油藏多孔介质中的吸附符合Freundlich吸附等温线。体积较大的微生物运移速度超前,说明采油微生物在多孔介质运移过程中存在不可及孔隙体积。采油微生物在多孔介质运移过程中受到平衡吸附和架桥筛分的共同作用,且架桥筛分的作用相比于平衡吸附更加突出。基于实验研究确定了微生物的迁移滞留机制,并在此基础上修改完善了微生物迁移数学模型。研究成果为微生物采油数值模拟软件的研发与优化提供了实验依据及理论基础。The microorganisms for oil recovery show obvious retention characteristics in porous media during migration process.Pseudomonas aeruginosa WJ-1 and Bacillus subtilis SLY-3 were taken as the research objects to study their migration and retention laws by isothermal adsorption experiment and flow experiment.The results show that the greater the specific surface area of porous media is,the more the adsorption sites will be and the greater the bacteria adsorption capacity will be.The bacteria adsorption capacity on porous media surface is certainly influenced by the hydrophobicity of bacteria and porous medium surface.The total bacterial concentration of microorganisms for oil recovery in the porous medium environment of reservoir is generally lower than 3.5×108cfu/mL.Under this condition,the adsorption of microorganisms for oil recovery in the porous medium is consistent with Freundlich adsorption isotherm model.The migration speed of larger microorganism is faster,thus proving the inaccessible pore volume of microorganisms for oil recovery during migration in the porous media.The microorganisms for oil recovery migrating in the porous media are affected by equilibrium adsorption and bridging screening,of which the bridging screening has more prominent effect than equilibrium adsorption.Based on experimental research,the migration and retention mechanism of microorganism was determined.On this basis,the mathematical model of microbial migration was modified and improved accordingly.The research results provided experimental and theoretical basis for the development and optimization of numerical simulation software for microbial oil recovery.

关 键 词：采油微生物 运移 多孔介质 吸附 滞留 数学模型 

分 类 号：TE357[石油与天然气工程—油气田开发工程]