WTX基因对结肠癌细胞增殖和凋亡的作用  

Effect of WTX gene on colon cancer cell proliferation and apoptosis

作　　者：曹梦园 白洋[2] 许颖[3] 

机构地区：[1]吉林省医学期刊社,长春130021 [2]吉林大学第一医院超声科,长春130021 [3]吉林大学第一医院肾内科,长春130021

出　　处：《重庆医学》2017年第2期145-147,共3页Chongqing medicine

基　　金：吉林省科技发展计划项目(2013052218JH)

摘　　要：目的 WTX基因转染结肠癌Lovo细胞株,分析WTX基因对结肠癌细胞增殖和凋亡的影响。方法将结肠癌Lovo细胞株分为WTX基因转染组和对照组,WTX基因转染组给予WTX质粒转染,对照组给予空载质粒转染。观察WTX基因转染组和对照组WTX基因的表达情况,两组Lovo细胞株结肠癌细胞的增殖情况、凋亡情况及细胞周期变化。结果 WTX基因转染组结肠癌细胞WTX蛋白表达明显高于对照组(P<0.05);WTX基因转染组结肠癌转染后4、7d光密度值均明显低于对照组(P<0.05);WTX基因转染组G1期细胞比例明显高于对照组(P<0.05),WTX基因转染组G2期和S期细胞比例明显低于对照组(P<0.05)。结论 WTX基因抑制结肠癌细胞的增殖,影响结肠癌细胞周期,对结肠癌细胞凋亡没有影响。Objective To use WTX gene for transfecting into Lovo colon cancer cell line,and to analyze the impact of WTX gene on colon cancer cell proliferation and apoptosis.Methods Lovo colon cancer cell line was divided into the WTX gene transfection group and control group.The WTX gene transfection group was given WTX plasmid transfection,while the control group received empty vector transfection.The expression of WTX gene in the WTX gene transfection group and control group was observed.The proliferation,apoptosis and cell cycle change of Lovo colon cancer cells in two groups were also observed.Results The WTX protein expression in the WTX gene transfection group was significantly higher than that in the control group（P〈0.05）;the OD values on 4,7dafter transfection in the WTX gene transfection group were significantly lower than those in the control group（P〈0.05）;the G1 phase cells proportion in the WTX gene transfection group was significantly higher than that in the control group（P〈0.05）,the G2 and S phase cells proportion in the WTX gene transfection group was significantly lower than that in the control group（P〈0.05）.Conclusion WTX gene inhibits the proliferation of colon cancer cells,affects colon cancer cells cycle,has no effect on apoptosis of colon cancer cells.

关 键 词：结肠肿瘤 基因 流式细胞术 细胞凋亡 WTX基因 结肠癌Lovo细胞株 细胞增殖 

分 类 号：R735.35[医药卫生—肿瘤]