长链非编码RNA BANCR在肝细胞癌组织中表达上调及其对患者疾病预后价值探讨  

Expression up-regulation of lncRNA BANCR in hepatocellular carcinoma tissue and its prognostic value on patients′disease

作　　者：赵冀[1] 周超[2] 李宏敏[3] 杨洪吉[1] 

机构地区：[1]四川省人民医院器官移植中心,成都610000 [2]四川省人民医院消化内科,成都610000 [3]四川省人民医院肿瘤科,成都610000

出　　处：《重庆医学》2017年第2期148-151,共4页Chongqing medicine

摘　　要：目的探讨BRAF激活的长链非编码RNA(BANCR)在肝细胞癌(HCC)组织中表达上调及其对患者疾病预后价值。方法采用实时定量PCR检测HCC组织和细胞系中BANCR表达情况,分析BANCR表达水平与患者临床病理学特征关系,采用四甲基噻唑蓝(MTT)、流式分析和肿瘤细胞体外侵入迁移分析探讨BANCR在HCC细胞中的生物学功能。结果与临近非肿瘤组织相比,HCC组织标本中BANCR表达水平明显上调(P<0.01),且HCC细胞系中BANCR表达水平也明显上调(P<0.05)。临床病理学特征分析结果提示BANCR表达升高与较高的肿瘤分级、肿瘤直径、静脉侵入及较高的TNM分期密切相关(P<0.05)。BANCR过表达HCC患者总生存期明显短于低表达患者(P<0.01)。多重变量Cox回归分析提示BANCR表达(RR=4.245,P=0.015)、肿瘤直径(RR=2.655,P=0.039)、静脉侵入(RR=3.278,P=0.022)和TNM分期(RR=6.379,P=0.006)是HCC患者总生存期独立预后因素。此外,Hep3B细胞中BANCR表达下调能明显抑制细胞侵入和转移,同时导致波形蛋白下调和E-钙黏蛋白上调。结论本研究结果表明BANCR可能参与HCC的发生和发展过程,可以作为HCC患者疾病预后标志物和临床治疗靶标。Objective To investigate the expression up-regulation of BRAF activated LncRNA（BANCR）in hepatocellular carcinoma（HCC）tissue and its prognostic value on patients′disease.Methods The quantitative real-time PCR（qRT-PCR）was used to detect BANCR expression in HCC tissues and cell lines.The association between BANCR expression level and clinicopathological features was also analyzed.3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT）,flow cytometry,and transwell invasion and migration assays were used to investigate the biological function of BANCR in the HCC cells.Results Compared with adjacent noncancerous tissues,BANCR expression was remarkably increased in HCC tissue sample（P 0.01）,moreover BANCR expression in HCC cell lines was also significantly up-regulated（P〈0.05）.The clinicopathologic features analysis revealed that the BANCR expression increase was closely correlated with higher tumor grade,tumor size,venous infiltration and higher TNM staging（P〈0.05）.The total survival period in HCC patients with BANCR overexpression was significantly shorter than that in the patients with BANCR low expression（P〈0.01）.The multi-variate Cox regression analysis indicated that the BANCR expression（RR=4.245,P=0.015）,tumor diameter（RR=2.655,P=0.039）,venous invasion（RR=3.278,P=0.022）and TNM staging（RR=6.379,P=0.006）were the independent prognostic factor of the total survival period in the HCC patients.Moreover,BANCR expression down-regulation in Hep3 Bcells could significantly inhibit the cellular invasion and transfer,meanwhile led to vimentin down-regulation and E-cadherin up-regulation.Conclusion This research results suggest that BANCR may contribute to HCC initiation and development process and would be used as a marker of the prognosis in HCC patients as well as a therapeutic target.

关 键 词：RNA 肝肿瘤 癌 肝细胞 长链非编码RNA BANCR 预后 

分 类 号：R735.7[医药卫生—肿瘤]