长链非编码RNA-UBC1对膀胱癌细胞生物学功能的影响  

Effect of long-chain non-coding RNA UBC1 on biological function of bladder cancer cells

作　　者：朱晨曦[1] 李文洲[1] 郭永连[1] 余家俊[1] 舒博[1] 陈琳[1] 李国灏[1] 卫丹[1] 

机构地区：[1]湖北省武汉市中心医院泌尿外科,430014

出　　处：《重庆医学》2017年第2期169-171,174,共4页Chongqing medicine

摘　　要：目的探讨长链非编码RNA(lncRNA)-尿路上皮癌相关基因1(UBC1)对膀胱癌细胞增殖、凋亡和侵袭转移的作用及其可能的机制。方法采用siRNA干扰技术沉默膀胱癌细胞系T24细胞的lncRNA-UBC1,设置阴性对照组,CCK-8检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell检测细胞侵袭能力,Western blot印迹法检测与转移相关的基质金属蛋白2(MMP-2)与zeste基因增强子同源2(EZH2)。结果 lncRNA-UBC1在T24细胞株中表达明显高于人胚胎膀胱组织来源细胞;与阴性对照组和空白对照组相比,沉默UBC1后,UBC1沉默组膀胱癌细胞增殖速率慢于对照组,UBC1沉默组膀胱癌细胞凋亡细胞比例高于阴性对照组[(18.72±7.79)%vs.(13.09±5.66)%,P<0.05],UBC1沉默组细胞侵袭能力受到抑制,T24细胞MMP-2与EZH2表达降低。结论沉默lncRNA-UBC1可抑制T24细胞增殖、凋亡及侵袭能力,其机制可能是通过MMP-2与EZH2途径。Objective To investigate the effect of long-chain non-coding RNA-UBC1（lncRNA-UBCI）on proliferation,apoptosis,invasion and metastasis of bladder cancer cells and its possible mechanism.Methods The siRNA was used to silence lncRNAUBC1 in bladder cancer cell line T24 cells.The negative control group was set.CCK-8was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,Transwell assay was used to detect the invasion ability and the expression of EZH2 and MMP-2was detected by Western blot.Results The lncRNA-UBC1 expression in T24 cell lines was significantly higher than that in human embryonic bladder tissue derived cells.Compared with the negative control group and blank control group,T24 bladder cancer cell proliferation rate in the UBC1 silence group was slower than that of the control groups.T24 bladder cancer cell apoptosis cell percentage in the UBC1 silence group was higher than that in the negative control group[（18.72±7.79）% vs.（13.09±5.66）%,P0.05].The T24 bladder cancer cell invasion ability in the UBC1 silence group was inhibited.Expression of MMP-2and EZH2 of T24cell in UBC1 silence group was decreased.Conclusion Silencing lncRNA-UBC1 can inhibit the proliferation,apoptosis and invasion ability of T24 cells and its mechanism may be through MMP-2and EZH2 pathway.

关 键 词：RNA 膀胱肿瘤 长链非编码RNA-UBC1 肿瘤浸润 细胞增殖 基质金属蛋白酶2 zeste基因增强子同源物2 

分 类 号：R737.14[医药卫生—肿瘤]