柚皮中的有效成分对人子宫颈癌Hela细胞增殖及凋亡的影响  

The effects of active ingredients in pomelo peel on the proliferation and apoptosis of Hela cell

作　　者：杜佳秋[1] 高嵩[2] 田婧依 王梦娇[2] 张泽嘉 郭家旺[2] 王芳芳[3] 马淑霞[3] 陈光[3] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154001 [2]佳木斯大学,黑龙江佳木斯154007 [3]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2016年第12期1393-1396,共4页Chinese Journal of Microecology

摘　　要：目的探讨柚皮素或柚皮苷对人子宫颈癌Hela细胞增殖及凋亡的影响。方法柚皮素或柚皮苷(0.250、0.125、0.063g/L)作用于人子宫颈癌Hela细胞后,MTT比色法检测Hela细胞增殖活性和半数抑制浓度(half maximal inhibitory concentration,IC50);流式细胞术检测柚皮素对Hela细胞凋亡的影响。结果柚皮素能抑制Hela细胞增殖,且呈剂量和时间依赖性,24、48和72hIC50值分别为0.24、0.11和0.07g/L;柚皮苷无抑制Hela细胞增殖的作用。柚皮素组(0.22g/L、0.11g/L、0.05g/L,作用48h)人子宫颈癌Hela细胞凋亡率高于对照组,差异有统计学意义(P=0.000239681,P=0.00012746,P=5.09328E-05,P<0.01)。结论柚皮中黄酮类提取物具有抑制人子宫颈癌Hela细胞增殖作用,其中柚皮素作用明显,部分作用机制与诱导肿瘤细胞凋亡有关。柚皮苷无抗人子宫颈癌作用。Objective To investigate the effects of naringenin and naringin on proliferation and apoptosis of human cervical carcinoma cell line HeIa cells. Methods Hela ceils were treated with naringenin and naringin respectively, then 3- （4,5-Dimethylthiazol-2-yl） -2,5-diphenyltetrazolium bromide （MTT） assay was used to test the inhibitory effects of naringenin and naringin （0. 250 g/L, 0. 125 g/L and 0. 063 g/L） on Hela cells in vitro and the half maximal inhibitory concentrations （IC50） were calculated. Fluorescence activating cell sorter （FACS） was used to detect the percentage of Hela cell apoptosis. Results Naringenin inhibited the proliferation of HeLa cells in a dose- and time-dependant way; The value of IC50 was 0.24 g/L at 24 hours, 0. 11 g/L at 48 hours and 0.07 g/L at 72 hours. But naringin did not inhibit the proliferation of He La cells. The percentage of HeLa cell apoptosis in naringenin group （0.22 g/L, 0. 11 g/L and 0.05 g/L, treated for 48 hours） was statistically higher than that in control group （P〈0.01） . Conclusion The flavonoid extracts from pomelo peel have anti-cancer activity in vitro, in which naringenin plays a leading role. The mechanism is partly related to the induction of the apoptosis of Hela cells. Naringin has no inhibitory effect on the proliferation of Hela cells.

关 键 词：柚皮 有效成分 HELA细胞 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]