YKL-40对卵巢癌SKOV-3细胞迁移能力的影响  

Regulation of epithelial ovarian carcinoma SKOV-3 cell migration by YKL-40

作　　者：陆衡[1] 刘延国[1] 李曙光[1] 陈晓康[1] 田琦[1] 衣翠华[1] 王秀问[1] LU Heng LIU Yanguo LI Shuguang CHEN Xiaokang TIAN Qi YI Cuihua WANG Xiuwen(Department of Medical Oncology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China)

机构地区：[1]山东大学齐鲁医院化疗科,山东济南250012

出　　处：《山东大学学报（医学版）》2017年第1期33-38,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81372530;81502615);山东大学齐鲁医院临床研究项目(2014QLKY30)

摘　　要：目的探讨YKL-40对卵巢癌SKOV-3细胞体外迁移能力的影响。方法采用RT-PCR检测卵巢癌SKOV-3细胞YKL-40 mRNA水平的表达;采用慢病毒感染的方法在SKOV-3细胞过表达YKL-40,qRT-PCR、ELISA、Western blotting验证YKL-40的过表达;Transw ell实验检测YKL-40过表达对SKOV-3细胞迁移能力的影响;特异性抑制剂抑制YKL-40过表达SKOV-3细胞中的p38 MAPK通路,检测其对细胞迁移能力的影响。结果 SKOV-3细胞中未检测到YKL-40 mRNA的表达;成功构建了YKL-40过表达细胞株LV-SKOV-3,感染率达90%以上;与空载体慢病毒感染的对照SKOV-3细胞(NC-SKOV-3)相比,LV-SKOV-3细胞YKL-40无论mRNA表达还是蛋白水平(细胞总蛋白、分泌蛋白)均明显上调;Transwell细胞迁移实验表明过表达YKL-40的LV-SKOV-3细胞较空载体对照细胞NC-SKOV-3迁移能力显著增强,而通过SB203580抑制p38 MAPK通路p38蛋白磷酸化后,LV-SKOV-3细胞的迁移能力明显下调。结论 YKL-40可显著促进卵巢癌SKOV-3细胞的迁移,这一作用可能是通过p38 M APK通路介导的。Objective To investigate the effect of YKL-40 on epithelial ovarian cancer SKOV-3 cell migration in vitro and to explore the underlying mechanism. Methods YKL-40 mRNA expression in SKOV-3 cells was detected by RTPCR. SKOV-3 cells were transfected with modified lentiviral vectors containing YKL-40 gene and YKL-40 overexpression was validated by qRT-PCR,ELISA and Western blotting. Transwell assay was used to evaluate the effect of YKL-40 on SKOV-3 cell migration. p38 MAPK pathway was repressed by specific inhibitors and its effect on YKL-40 overexpressed SKOV-3 cell migration was further investigated. Results YKL-40 mRNA was not detected in SKOV-3 cells.After lentivirus transfection and puromycin selection,YKL-40 overexpressed cell line LV-SKOV-3 and negative control cell line NC-SKOV-3 were established. Further analyses confirmed YKL-40 was significantly increased in LV-SKOV-3cells on both mRNA and protein levels compared to NC-SKOV-3. After YKL-40 overexpression,the migration ability of LV-SKOV-3 was enhanced while LV-SKOV-3 cell migration was decreased after inhibition of p38 MAPK pathway via SB203580. Conclusion YKL-40 significantly promotes the migration ability of SKOV-3 cells,which is partly mediated by p38 MAPK pathway.

关 键 词：卵巢癌 YKL-40 细胞迁移 P38 MAPK通路 

分 类 号：R737.31[医药卫生—肿瘤]