肝X受体在卵巢癌组织中的表达及T0901317在卵巢癌细胞系SKOV3中的作用  

Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line

作　　者：赵路[1] 矫俊 张腾[1] 焦薪霖 崔保霞[1] ZHAO Lu JIAO Jun ZHANG Teng JIAO Xinlin CUI Baoxia(Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China)

机构地区：[1]山东大学齐鲁医院妇产科,山东济南250012

出　　处：《山东大学学报（医学版）》2017年第1期49-53,共5页Journal of Shandong University:Health Sciences

基　　金：山东省重点研发计划(2015GGE27352)

摘　　要：目的检测肝X受体蛋白在卵巢癌组织及正常卵巢组织中的表达,探讨肝X受体激动剂T0901317对卵巢癌细胞系SKOV3细胞活力及凋亡的影响。方法收集2015年8月至2016年8月行初次手术治疗的51例卵巢癌患者的组织标本,另选取32例因子宫肌瘤等良性病变切除的正常卵巢组织作对照。采用免疫组织化学方法检测肝X受体蛋白在卵巢癌组织及正常卵巢组织中的表达;CCK8检测T0901317对卵巢癌细胞系SKOV3增殖的影响;流式细胞术检测T0901317对卵巢癌细胞系SKOV3凋亡的影响。结果肝X受体蛋白在正常卵巢组织较卵巢癌组织表达量高,两组比较差异有统计学意义(P<0.001),T0901317抑制卵巢癌细胞系SKOV3增殖具有明显的剂量依赖性和时间依赖性(P<0.05),T0901317促进SKOV3凋亡具有明显的剂量依赖性(P<0.05)。结论肝X受体表达下调可能与卵巢癌的发生发展有关,激活肝X受体可能会成为卵巢癌潜在的治疗靶点。Objective To detect the expression of liver X receptor α（ LXRα） in normal ovarian tissues and ovarian cancer tissues,and to investigate the effects of the LXRs agonist T0901317 on SKOV3 cell line. Methods A total of51 cases of ovarian cancer treated in our hospital during Aug. 2015 and Aug. 2016 were enrolled. Another 32 cases of benign ovarian lesions served as controls. The expression of LXRα was detected with immunohistochemistry. The proliferation of SKOV3 cell line treated with T0901317 was detected with CCK8 assay. The effect of T0901317 on the apoptosis of SKOV3 cell line was assayed with flowcytometry. Results There was significant difference in LXRα expression between normal ovarian tissues and ovarian cancer tissues（ P〈0. 001）. T0901317 significantly inhibited the proliferation of SKOV3 cell line in a dose- and time- dependent manner（ P〈0. 05）. Activation of LXRs could induce the apoptosis of SKOV3 cell line significantly（ P〈0. 05）. Conclusion The down-regulation of LXRs receptor may be related to the pathogenesis of ovarian cancer. For advanced ovarian cancer,activating LXR pathway is therefore a potential adjuvant therapy.

关 键 词：卵巢肿瘤 肝X受体 T0901317激动剂 增殖 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]