大蒜素对荷子宫内膜癌裸小鼠移植瘤生长的影响及其机制研究  

Effects and Mechanism of Allitridi on Xenografts Growth in Endometrial Cancer of Nude Mice

作　　者：贾丽[1] 

机构地区：[1]邯郸市中心医院妇二科,056001

出　　处：《医学研究杂志》2017年第1期120-123,共4页Journal of Medical Research

摘　　要：目的研究大蒜素(allitridi)对荷子宫内膜癌裸小鼠移植瘤生长的影响及其机制。方法取对数生长期子宫内膜癌Ishikawa细胞,皮下注射接种的方法制备荷子宫内膜癌裸小鼠模型,成瘤后取60只裸小鼠模型随机分为模型组、大蒜素高(40mg/kg)、中(20mg/kg)、低(10mg/kg)剂量组和顺铂2mg/kg组,每组12只;各治疗组均隔天腹腔注射给药1次,共给药治疗5次。治疗完成后剥取肿瘤组织并称量、计算抑瘤率;HE染色法观察肿瘤组织形态学变化;TUNEL染色法观察肿瘤组织细胞凋亡状况并计算凋亡指数(apoptosis index,AI),免疫组织化学法(immunohistochemistry,IHC)观察肿瘤组织bcl-2、Bax、caspase-3蛋白表达并进行半定量分析,计算Bax/bcl-2比值。结果大蒜素各组和顺铂组裸小鼠移植瘤肿瘤组织细胞呈皱缩、片状坏死等病理性改变,凋亡细胞数量明显增多,以大蒜素高剂量组最为显著。与模型组比较,大蒜素高、中剂量组和顺铂组瘤体重量显著减轻(P<0.05,P<0.01),抑瘤率显著升高(P<0.01),肿瘤组织bcl-2蛋白表达显著下调(P<0.05,P<0.01),Bax和caspase-3蛋白表达显著上调(P<0.05,P<0.01),Bax/bcl-2表达比值显著升高(P<0.01)。与顺铂组比较,大蒜素高剂量组Bax/bcl-2表达比值显著升高(P<0.05)。结论大蒜素对荷子宫内膜癌裸小鼠移植瘤生长具有抑制作用,其机制可能与大蒜素能够下调bcl-2表达、上调Bax和caspase-3表达、提高Bax/bcl-2表达比值进而促进肿瘤细胞凋亡有关。Objective To investigate the effects and mechanism of allitridi on xenografts growth in endometrial cancer of nude mice. Methods Nude mice models bearing human endometrial cancer were made by injecting inoculation human endometrial cancer Ishikawa cell line were divided randomly into five groups ： model group, allitridi high - dose （40mg/kg） , middle - dose （20mg/kg） , low - dose （lOmg/kg） groups and cisplatin 2mg/kg group（n = 12）. The drugs were given by intraperitoneal injection for 5 times, once every two days. The histopathological changes of tumor tissue was observed by HE staining,and the tumor cells apoptosis was detected by TUNEL. The expression of bcl- 2, Bax, caspase -3 protein were detected by IHC and were semi - quantitative analysised. Results Compared with model group, the necrosis presentation, tumor cell shrinkage and other pathological morphological changes appeared, the number of apoptotic cells in tumor tissue was significantly increased, especially the rats in allitridi high - dose group. The tumor weight of allitridi high - dose and middle - dose groups were significantly decreased and the inhibition rate were significantly increased. The expression of bcl - 2 protein was significantly decreased （ P 〈 0.05, P 〈 O. 01 ） , the expression of Bax and caspase - 3 were significantly increased（ P 〈 0. O1 ） , and the ratio of Bax/bcl - 2 were significantly increased（P 〈 0.01 ）. Compared with cisplatin group, the ratio of Bax/bcl - 2 in allitridi high - dose group was significantly increased（P 〈 0.05）. Conclusion Allitridi had inhibitive effects on xenografts growth in en- dometrial cancer of nude mice, which perhaps related to its effects of down - regulating the expression of bcl - 2 protein, up - regulating the expression of Bax and easpase - 3 protein, increasing the ratio of Bax/bel - 2, promoting endometrial cancer cell apoptosis.

关 键 词：大蒜素 子宫内膜癌 裸小鼠 移植瘤 影响 

分 类 号：R285.5[医药卫生—中药学]