p-JNK/p-c-Jun通路参与大鼠杏仁核点燃癫痫模型  

Involvement of p-JNK/p-c-Jun signaling in Amygdala-kindled Seizures

作　　者：李巷[1] 潘建青[1] 康慧聪[2] 杨志刚[1] 韩金玲[1] 聂荔[1] 肖小华[3] 张玲玲[1] 朱遂强[2] LI Xian PAN Jian-qing KANG Hui-cong YANG Zhi-gang HAN Jin-ling NIE Li XIAO Xiao-hua ZHANG Ling-ling ZHU Sui-qiang(Department of neurology, Nanshan Hospital, Shenzhen, Guangdong, 518052, China Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Teclmology, Wuhan, 430030, China Department of Neurology, Second People＇s Hospital, Shenzhen, Guangdong, 518052, China)

机构地区：[1]深圳市南山区人民医院神经内科,广东深圳518052 [2]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [3]深圳市第二人民医院,广东深圳518052

出　　处：《神经损伤与功能重建》2016年第6期473-475,共3页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金青年基金(No.81201006);湖北省自然科学基金(No.2011CDB201);深圳市南山区技术研发和创意设计项目分项基金(No.南科研卫2012002);深圳市南山区技术研发和创意设计项目分项基金(No.南科研卫2014015);深圳市科技计划项目(No.JCYJ20140414170821276)

摘　　要：目的:研究杏仁核点燃癫痫模型中c-Jun氨基末端激酶(JNK)通路的活化。方法:雄性Wistar大鼠20只,随机分为对照组10只和模型组10只。将电极植入大鼠右侧杏仁核,对照组不给予电刺激,模型组每天给予500μA的电流刺激,大鼠连续10 d在刺激后达到5级发作视为点燃成功。成功点燃的大鼠,在最后1次5级发作后2 h处死。Western blot检测2组海马JNK、p-JNK、c-Jun、p-c-Jun的表达。结果:模型组刺激侧海马p-JNK、p-c-Jun表达明显高于对照组(P<0.05)。结论:p-JNK/p-c-Jun通路可能参与杏仁核癫痫模型点燃。Objective： To evaluate the involement of JNK mitogen-activated protein kinase signaling pathway in amygdala-kindled models . Methods： Twenty Wistar rats were randomly divided into control group （n=10） and kindling group （n=10）. Electrodes were implanted into the right amygdala of all the rats. Kindling was accomplished using stimulus strength of 500 μA applied daily to the amygdala until stage 5 were induced in rats for consecutive 10 days. All kindled rats were decapitated 2 h after the tenth stage 5 seizures. Rats in the control group did not receive stimulus. Western blot were performed to test the expression of JNK, p-JNK, c-Jun, p-c-Jun in hippocampus. Results： Kindling increased the expression of p-JNK and p-c-Jun in amygdala-kindled models. The difference is statistical significant between control group and kildling group. Conclusion： p-JNK/p-c-Jun signaling may be involved in amygdala-kindled seizures.

关 键 词：杏仁核点燃 磷酸化JNK 磷酸化c-Jun 

分 类 号：R741[医药卫生—神经病学与精神病学] R742.1[医药卫生—临床医学]