颅脑损伤患者外周血miRNA-124与Treg细胞和IL-10表达的关系  

Relationship among Mi RNA-124,Treg Cells and IL-10 expression in the Peripheral Blood of Patients with Traumatic Brain Injury

作　　者：周贵勤 陈新寿 师宁[2] 田冰锋[3] ZHOU Gui-qin CHEN Xin-shou SHI Ning TIAN Bing-feng(Department of Neurosurgery, Forest Industry Professional Hospital, Shanxi 716000, China Department of Neurosurgery, Affiliated Hospital of Yah An university, Shunxi 716000, China Department of Neurosurgery, Hanzhong Central Hospital, Hanzhong 723000)

机构地区：[1]陕西省森林工业职业医院神经外科,西安710031 [2]延安大学附属医院神经科,陕西延安716000 [3]汉中市中心医院神经外科,汉中723000

出　　处：《神经损伤与功能重建》2016年第6期518-520,共3页Neural Injury and Functional Reconstruction

摘　　要：目的:研究颅脑损伤后外周血小RNA(miRNA)-124与调节性T细胞(Treg)及白细胞介素-10(IL-10)表达的关系,探讨miRNA-124在颅脑损伤中的作用及其机制。方法:纳入颅脑损伤患者109例,根据GCS评分分为轻度损伤组35例,中度损伤组45例,重度损伤组29例,同时纳入正常体检者20例为对照组。受伤后24 h分别采集各组的外周静脉血,采用流式细胞术检测Treg细胞的表达;实时荧光定量聚合酶联反应检测miRNA-124表达;酶联免疫吸附试验检测IL-10的水平。结果:各组颅脑损伤患者外周血Treg比例、miR-124表达及IL-10水平均显著高于对照组(P<0.05),中、重度颅脑损伤组高于轻度损伤组(P<0.05),重度组高于中度组(P<0.05);重度损伤组患者外周血miR-124和Treg比例呈正相关。结论:颅脑损伤后,miRNA-124可能参与对Treg细胞增殖的调节,调控炎症反应。Objective： To evaluate the relationship among micro RNA（miRNA）-124, T regulatory cells（Treg）and interleukin（IL）-10 levels in the peripheral blood of patient with traumatic brain injury. Methods： One hundred and nine patients with traumatic brain injury were enrolled and divided into mild group（n=35）, moderate group（n=45） and severe group（n=29） according to their Glasgow coma scale（GCS） scores. Twenty healthy volunteers were enrolled into controls. The level of miRNA-124, Treg cells and IL-10 were calculated by RT-PCR,flow cytometry and ELISA respectively. Results： The levels of miRNA-124, Treg cells and IL-10 in the mild,moderate and severe groups were significantly higher than those in the controls（P〈0.05）. The levels of miRNA-124, Treg cells and IL-10 in the mild group were lower than those in the moderate and severe groups（P〈0.05）. In the severe group, miRNA-124 is positively correlated with Treg cell and IL-10. Conclusion： miRNA-124 could regulate Treg cells and the inflammation response which play an important role in the recovery process of traumatic brain injury.

关 键 词：颅脑创伤 微小RNA-124 调节性T淋巴细胞 白细胞介素-10 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]