凡德他尼诱导甲状腺癌细胞SW579凋亡及其机制  

Vandetanib induced apoptosis in thyroid cancer cells SW579 and its mechanisms

作　　者：王巍[1] 宋晓雯[1] 邵跃[1] 赵成海[1] Wang Wei Song Xiaowen Shao Yue Zhao Chenghai(Department of Pathophysiology, College of Basic Medical Science, China Medical University, Liaoning Shenyang 110122, China.)

机构地区：[1]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2016年第23期3703-3705,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81502107);辽宁省自然科学基金项目(编号:2014021036)

摘　　要：目的:检测凡德他尼对甲状腺癌SW579细胞的作用及其机制。方法:采用MTT比色法检测凡德他尼处理后SW579细胞的增殖情况。利用流式细胞技术检测SW579细胞的凋亡比例。为了检测凡德他尼对SW579细胞作用的机制,利用小分子对接技术初步模拟可能的信号传导通路。利用Western blot技术证实模拟的细胞信号传导通路。结果:凡德他尼可以明显抑制SW579细胞增殖,并且诱导凋亡。在凋亡过程中,凡德他尼能够结合Smad3并激活Smad传导通路。结论:凡德他尼通过Smad传导通路导致SW579细胞凋亡。Objective： To study effects and mechanisms of vandetanib in thyroid cancer cell SW579. Methods： The proliferative ratio of SW579 cells after treated with vandetanib was detected by MTT. The apoptotic ratio of SW579 cells after treated with vandetanib were analyzed by FCM. In order to detect the mechanisms of vandetanib in SW579 cells,small molecular docking technology was used as a preliminary simulation of signaling pathway. Then the results of simulation were confirmed by Western blot. Results： The proliferative ratio of SW579 cells was inhibited significantly by vandetanib. The Smad signaling pathway was activated by vandetanib. Conclusion： Vandetanib induced apoptosis in SW579 cells through Smad signaling pathway.

关 键 词：SW579细胞 凡德他尼 凋亡 小分子对接技术 SMAD3 

分 类 号：R730.53[医药卫生—肿瘤]