硫胺素水平对团头鲂幼鱼生长、肝组织沉积量及血液生化指标的影响  

EFFECTS OF DIETARY THIAMIN LEVELS ON GROWTH, HEPATIC THIAMIN CONTENTS AND PLASMA BIOCHEMICAL INDEXES OF JUVENILE BLUNT SNOUT BREAM, MEGALOBRAMA AMBLYCEPHALA

作　　者：李鹏飞[1] 王莹[1] 蒋广震[1] 王菲[1] 刘文斌[1] 

机构地区：[1]南京农业大学动物科技学院,江苏省水产动物营养重点实验室,南京210095

出　　处：《水生生物学报》2017年第1期108-113,共6页Acta Hydrobiologica Sinica

基　　金：现代农业产业技术体系建设专项-国家大宗淡水鱼类产业技术体系资金(No:CARS-46-20);农业公益性行业科研专项-水产养殖动物营养需求与高效配合饲料开发(No:201003020)资助~~

摘　　要：为研究硫胺素对团头鲂幼鱼生长、组织沉积量和血液生化指标的影响,试验采用单因素浓度梯度设计,配制了6组等氮等能的半纯合饲料,各组硫胺素含量分别为0、0.51、0.98、1.59、2.13和2.68 mg/kg。选取团头鲂幼鱼720尾[初重为(0.30±0.01)g],按随机原则分为6组,每组4重复,各重复30尾,日投饵3次,饲喂8周后采集样品。结果表明,与对照组相比,0.98、1.59、2.13、和2.68 mg/kg添加组的增重率和特定生长率均显著提高(P<0.05)。1.59和2.13 mg/kg硫胺素添加组的成活率显著高于对照组(P<0.05)。随着饲料中硫胺素含量的升高,血浆葡萄糖含量呈现先下降后上升的趋势,血浆葡萄糖水平在1.59 mg/kg时为最小值(P<0.05)。对照组与0.51 mg/kg硫胺素组相比血浆中丙酮酸含量差异不显著,但显著高于其他试验组(P<0.05)。以团头鲂幼鱼的增重率和肝脏硫胺素沉积量为评价指标,进行双折线回归分析,饲料中硫胺素适宜添加水平分别为1.48和1.84 mg/kg。This study was conducted to determine the optimal dietary thiamine requirement of juvenile blunt snout bream （Megalobrama amblycephala） on the basis of growth parameters, hepatic thiamine contents and plasma bio-chemical indexes. 720 fishes [average initial weight：（0.30±0.01） g] were fed six isonitrogenous and isoenergetic diets containing different thiamine levels （0, 0.51, 0.98, 1.59, 2.13and 2.68 mg/kg） 3 times daily for 8 weeks, respectively. Each diet was tested in four replicates, and each replicate holds 30 fish. The results showed that both the weight gain and specific growth rate of fish at 0.98, 1.59, 2.13 and 2.68 mg/kg groups were significantly higher than the control group （P〈0.05）. Survival rate increased significantly as dietary thiamin levels increased from 0 to 2.13 mg/kg （P〈0.05）. Plasma glucose level decreased significantly （P〈0.05） as dietary thiamine levels increased from 0 to 1.59 mg/kg, but it increased significantly （P〈0.05） with further increased thiamine levels. The addition of thiamine except 0.51 mg/kg group significantly increased plasma pyruvate level （P〈0.05）. On the basis of the broken-line analysis of weight gain rate and hepatic thiamin contents, the optimal dietary thiamin requirement of juvenile blunt snout bream was 1.48 and 1.84 mg/kg, respectively.

关 键 词：团头鲂 硫胺素 生长 肝脏硫胺素沉积量 血液生化 

分 类 号：S963.73[农业科学—水产养殖]