ARHI基因与HuR蛋白在胰腺癌PANC-1细胞中的表达  

Expression of ARHI and HuR in PANC-1 Cells

作　　者：杨红[1] 胡珊珊[1] 阮戈冲 杨晓鸥[1] 钱家鸣[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院消化内科,北京100730

出　　处：《协和医学杂志》2017年第1期39-42,共4页Medical Journal of Peking Union Medical College Hospital

基　　金：国家自然科学基金(81072055);国家教育部博士点专项基金-新教师基金(20101106120059)

摘　　要：目的观察ARHI基因转染胰腺癌PANC-1细胞后对HuR蛋白的影响。方法采用脂质体法将表达ARHI基因的质粒p LNCX2-ARHI-EGFP和空载体p LNCX2-EGFP转染胰腺癌PANC-1细胞,细胞分为3组:实验组(p LNCX2-ARHIEGFP)、空载体转染组(p LNCX2-EGFP)和PANC-1细胞空白对照组,用G418筛选稳定转染的细胞株;采用PCR和Western blot方法检测ARHI基因表达,采用Western blot方法检测HuR蛋白表达。结果稳定转染ARHI基因组可检测到ARHI基因mRNA和蛋白的表达。根据图像灰度计算蛋白条带相对表达量,稳定转染ARHI基因组HuR蛋白表达(0.2226±0.0644)较空载体对照转染组(0.4063±0.0925,P=0.029)和PANC-1细胞空白对照组(0.4178±0.1319,P=0.022)显著降低。结论 ARHI基因可以导致胰腺癌细胞株中HuR蛋白表达降低。Objective To investigate the effect of ARHI transfection on the expression of HuR protein in PANC-1 cells. Methods Plasmids pLNCX2-ARHI-EGFP which express ARHI and empty plasmid pLNCX2-EG- FP were used to transfect PANC-1 cells, which were divided into three groups： pLNCX2-ARHI-EGFP group, pLNCX2-EGFP group, and blank control. G 418 was used to select stable transfected cell lines. ARHI gene ex-pression was detected by PCR and Western blot, and the expression of HuR protein was detected by Western blot. Results PANC-1 cells transfected with pLNCX2-ARHI- EGFP, the mRNA and protein expression of ARHI were detected. HuR protein expression appeared significantly decreased in PANC- 1 cells transfected with pLNCX2-ARHI-EGFP （0.2226 ± 0.0644） compared with the pLNCX2-EGFP-transfected cells （0.4063 ± 0.0925, P = 0.029） and PANC-1 blank control cells （0.4178 ±0. 1319, P = 0.022）. Conclusion ARHI gene in PANC-1 cells may reduce the expression of HuR protein.

关 键 词：胰腺癌 ARHI基因 HUR 

分 类 号：R34[医药卫生—基础医学]