NaCl和Na2SO4胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析  

Effects of NaCl and Na_2SO_4 stress on germination of winter rapeseed(Brassica rapa L.) and analysis of salt resistance

作　　者：王治江[1] 刘自刚[1] 孙万仓[1] 方彦[1] 武军艳[1] 刘海卿[1] 赵艳宁[1] 蒲媛媛[1] 袁金海[1] 钱武[1] 陈奇[1] 方园[1] 徐梦雪[1] 

机构地区：[1]甘肃省油菜工程技术研究中心,甘肃省干旱生境作物重点实验室,甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070

出　　处：《干旱地区农业研究》2016年第6期243-252,共10页Agricultural Research in the Arid Areas

基　　金：国家现代农业产业技术体系建设专项资金(CARS-13);国家自然科学基金(31460356);国家863计划(2011AA10A104);国家973计划(2015CB150206);国家农业科技成果转化项目(2014G10000317)

摘　　要：以6个耐盐性不同的白菜型冬油菜种子为材料,将NaCl和Na_2SO_4按摩尔比1:1混合,设浓度为0(CK)、45、90、135、180、240 mmol·L^(-1)6个处理,测定其对种子萌发和生长参数的影响,研究不同冬油菜种子萌发时的耐盐能力,以期为耐盐冬油菜的筛选提供依据。结果表明:(1)45 mmol·L^(-1)和90 mmol·L^(-1)盐浓度对种子萌发的影响不明显;135 mmol·L^(-1)和180 mmol·L^(-1)盐浓度明显抑制了种子萌发,表现为种子发芽势、发芽率、发芽指数、活力指数、胚根长、胚芽长、鲜重和干重均随盐浓度的增大而明显降低。因此,135~180 mmol·L^(-1)盐浓度可认为是耐盐性鉴定的适宜范围。(2)不同指标对盐胁迫的敏感程度差异较大,发芽指数、活力指数、胚芽长和鲜重与盐浓度均为极显著负相关,对盐胁迫最为敏感。(3)3个耐盐冬油菜ky-1、ky-10-191和ky-NDJ的种子发芽指数、活力指数、胚芽长和鲜重的耐盐临界值显著高于原有品系平油1号、10-191和ky-NDJ。ky-1、ky-10-191、ky-NDJ的耐盐临界值分别为133.895、123.264、107.399 mmol·L^(-1),而平油1号、10-191、NDJ的耐盐临界值分别为117.394、103.947、93.834 mmol·L^(-1)。用隶属函数法综合评价,耐盐性强弱顺序依次为ky-1>ky-10-191>平油1号>kyNDJ>10-191>NDJ。可见,ky-1、ky-10-191和ky-NDJ的耐盐性较平油1号、10-191和NDJ均有所提高。Winter rapeseeds＇（Brasska rapa L.） seeds from six cultivars was treated by mixed solution of NaCl and Na2SO4（mole ratio of 1：1） to investigate the salt tolerance for screening of salt tolerance winter rapeseed.The concentration of mixed solution included 0（CK）,45,90,135,180,240 mmol·L^-1.Seed germination and growth parameters were determined.The results showed that salt concentration of 45 mmol·L^-1 and 90 mmol·L^-1 had little effect on seed germination but 135 mmol·L^-1 and 180 mmol·L^-1 significandy inhibited the germination of seeds,germination potential,germination rate,germination index,vigor index,length of radicle and plumule,fresh weight and dry weight,indicating that salt concentration of 135 - 180 mmol·L^-1 could be considered as the appropriate concentration of salt resistance identification.Moreover,the salt stress sensitivity of these indicators differenced a lot.Germination index,vigor index,plumule length and fresh weight and salt concentration were negatively correlated,suggesting that they were the most sensitive indicators.The salt tolerance critical value of ky- 1,ky- 10- 191 and ky- NDJ were 133.895,123.264,107.399 mmd·L^-1,while that of Pingyou 1,10-191 and NDJ were 117.394,103.947,93.834 mmol·L^-1,respectively.The former was significandy higher than the latter.Comprehensive evaluation indicated that the salttolerance rank of six winter rapeseeds was ky-1 ky-10-191 Pingyou 1 ky- NDJ 10-191 NDJ.

关 键 词：NACL NA2SO4 盐胁迫 白菜型冬油菜 种子萌发 耐盐性 

分 类 号：S565.4[农业科学—作物学] S503.4