高效苯酚降解菌PDB1的筛选及降解特性研究  

Breeding of Phenol-degradation Bacteria and Study on Phenol Biodegradation by the Strain PDB1

作　　者：王图锦[1] 潘瑾[1] 刘雪莲[1] WANG Tu-jin PAN Jin LIU Xue-lian(School of River and Ocean E nginee ring, Chongqing Jiaotong University, Chongqing 400074 , P. R. Chin)

机构地区：[1]重庆交通大学河海学院,重庆400074

出　　处：《科学技术与工程》2017年第2期301-304,共4页Science Technology and Engineering

基　　金：重庆市基础与前沿研究计划项目(cstc2014jcyjA20011)资助

摘　　要：从一个焦化厂受污染土壤中分离获得一株具有高效降解苯酚能力的不动杆菌属细菌PDB1,以苯酚为唯一碳源和能源对该菌株降酚特性进行研究。结果表明:在初始苯酚浓度低于1 200 mg/L时,该菌株能够在60 h内完全降解苯酚;在更高苯酚浓度下,高浓度苯酚对菌株生长造成明显抑制,菌株降酚能力出现显著下降。对该菌株苯酚降解动力学过程进行模拟,苯酚降解符合基质抑制型的Haldane模型;当初始苯酚浓度低于144.56 mg/L时,苯酚比降解速率随苯酚浓度增大而增大;在苯酚浓度为144.56 mg/L时,得到最大比降解速率,苯酚浓度高于144.56 mg/L时,苯酚比降解速率逐渐下降。该菌株降解苯酚最适温度为25~40℃,p H为7.0~8.0,菌株具有良好的高盐分耐受性,能够耐受5%Na Cl浓度并取得良好降酚效果。A bacterial strain which could degrade phenol with high efficiency was isolated from the polluted soils in a coke plant. The strain was named PDB1 and was identified as the genus Acinetobacter sp with 16 S rRNA gene sequence. Biodegradation was investigated in details with phenol as the sole carbon and energy source. The results indicated that PDB1 could utilize phenol as a sole source of carbon and could degrade less than 1 200 mg / L phenol completely in 60 h,but higher phenol concentrations had an inhibitory effect. The process of phenol degradation was investigated using Haldane kinetic model. Phenol degradation kinetic studies indicated that the degradation followed Haldane＇s model. The optimal concentration of initial phenol was 144. 56 mg / L. The rate of phenol degradation increased with increasing initial phenol concentrations up to 144. 56 mg / L. Further increase in initial phenol concentrations caused decrease in the rate of phenol degradation because of substrate inhibition. The optimal conditions for biodegradation of phenol were at 25 - 40 ℃,p H 7. 0 - 8. 0 and 0 - 5% Na Cl.

关 键 词：苯酚 生物降解 降解动力学 抑制 

分 类 号：X172[环境科学与工程—环境科学]