清化凉血化瘀方对急性肝衰竭大鼠生存率及生化指标的影响  

Effect of Qinghua Liangxue Huayu Decoction on the survival rate and biochemical indexes of acute liver failure rats

作　　者：虞胜[1] 祝峻峰[1] 陈建杰[2] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海中医药大学附属曙光医院,上海200021

出　　处：《现代中西医结合杂志》2017年第3期234-237,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：上海市进一步加快中医药事业发展三年行动计划(ZY3-JSFC-1-1017);上海市卫生和计划生育委员会中医药科研专项课题(2016LP035)

摘　　要：目的观察清化凉血化瘀方对急性肝衰竭大鼠肝功能和累积生存率的影响。方法将160只大鼠随机分为空白组20只、模型组35只、西药组35只、中药组35只、中西医结合组35只,西药组和中西医结合组予复方甘草酸苷溶液灌胃,中药组予清化凉血化瘀方灌胃,空白组与模型组予灭菌蒸馏水灌胃。灌胃3 d后,除空白组外,其余组均腹腔注射纯D-氨基半乳糖(D-Gal)800 mg/kg、内毒素脂多糖(LPS)8μg/kg制造急性肝衰竭模型,造模后西药组和中药组再分别给予相应药物灌胃1次,中西医结合组予清化凉血化瘀方灌胃1次。造模24 h后,将每组大鼠再随机分为A、B 2个亚组,空白组2个亚组均为10只,其余每组中A组20只,B组15只,处死每组中A组大鼠,采集血清,检测肝功能、凝血功能;每组中B组大鼠观察至96 h,统计大鼠累积生存率及生存时间。结果模型组大鼠血清ALT、AST、TBil、CHE水平及血浆PT均明显高于空白组(P均<0.05),ALB明显低于空白组(P<0.05);各给药组大鼠血清ALT、AST、TBil、CHE水平及血浆PT均明显低于模型组(P均<0.05),ALB均明显高于模型组(P均<0.05);中西医结合组和西药组ALT、AST水平均明显低于中药组(P均<0.05),ALB水平均明显高于中药组(P均<0.05),且中西医结合组ALB水平明显高于西药组(P均<0.05);中西医结合组TBil、CHE水平及血浆PT明显低于中药组及西药组(P均<0.05),西药组CHE水平明显低于中药组(P均<0.05)。各给药组大鼠存活率均明显高于模型组(P均<0.05),但各给药组间比较差异均无统计学意义(P均>0.05)。各组生存时间比较差异均有统计学意义(P均<0.05);中西医结合组生存时间明显长于模型组(P<0.05)。结论清化凉血化瘀方联合复方甘草酸苷可改善肝衰竭大鼠的肝功能及凝血功能,提高大鼠累积生存率,延长大鼠生存时间。Objective It is to observe the influence of Qinghua Liangxue Huayu decoction（QLHD） on liver function and survival rate in rats with acute liver failure. Methods 160 rats were randomly divided into normal group（20 cases）,model group（35 cases）,TCM group（35 cases）,WM group（35 cases）,and combined group（35 cases）,then the animals were treated by fill the stomach with sterilized distilled water in normal group and model group,with QLHD in TCM group,with Stronger Neo-Minophagen C in WM group,and with QLHD plus Stronger Neo-Minophagen C in combined group. After treated for 3 days,all rats except the normal group were treated with intraperitoneal injection of pure D-galactose 800 mg/kg and endotoxin lipopolysaccharide 8μg/kg to produce acute liver failure model,then the animals were treated with QLHD in TCM group and combined group,and with Stronger Neo-Minophagen C in WM group for 1 time. 24-hour After the model was built,the rats in each group were randomly divided into 2 subgroups of A and B,there were 10 cases in each subgroups of normal group,and 20 cases in A subgroup and 15 cases in B subgroup in the other groups. The rats in A subgroup were killed and blood was collected to detect liver function and coagulation function; and the rats in B subgroup were observed for 96-hour after the model was built to count the cumulative survival rate and survival time of rats. Results The serum levels of ALT,AST,TBil,CHE and plasma PT in model group were significantly higher than those in normal group（all P〈0. 05）,but the level of ALB was significantly lower than that in normal group（P〈0. 05）; the serum levels of ALT,AST,TBil,CHE and plasma PT in TCM group,WM group and combined group were significantly lower than those in model group（all P〈0. 05）,but the level of ALB was significantly higher than that in model group（P〈0. 05）; the levels of ALT and AST in the combined group and WM group were significantly lower than those in TCM group（all P〈0. 05）,but the level of ALB was sig

关 键 词：清化凉血化瘀方 急性肝衰竭 脂多糖 氨基半乳糖 复方甘草酸苷 

分 类 号：R-332[医药卫生]