PDGF-A基因修饰皮片对眼睑皮肤创伤修复的研究  

PDGF-A gene modification in eyelid skin wound repair

作　　者：汪峰[1] 阎国和[2] 徐宁[3] 

机构地区：[1]中国人民解放军458医院眼科,广州市510610 [2]第三军医大学预防医学院全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆市400038 [3]广州市第八人民医院眼科,广州市510000

出　　处：《老年医学与保健》2016年第6期348-351,共4页Geriatrics & Health Care

基　　金：国家重点基础研究发展规划(973项目)(G1999054205);国家自然科学基金(30200142)

摘　　要：目的利用PDGF-A基因修饰的全层皮肤皮片，利用HE染色、PCR、蛋白定量分析几个层面，探讨其对眼睑皮肤损伤修复的情况及部分机理。方法贵州小香猪6只，背部脊柱两侧造成全层皮肤缺损圆形创面（Ф3．67cm）各3个，共36个创面。A组（基因修饰皮片组）“PDGF-A基因修饰皮片”，移植到其右侧的18个创面作为实验组；B组（细胞皮片植入组）以HAM负载未经基因修饰的自体bMSCs与角朊细胞，构建成“细胞皮片”植入其左侧前6个创面；C组（单纯HAM敷盖组）单纯HAM敷盖其左侧后12个创面，作对照组。观察移植后1～4周内，各组创面愈合、肉芽组织生长、上皮化等情况。并进行创面组织HE、Laminin免疫组化及胶原含餐等检测。结果（1）A组于伤后15～17d愈合，B组于伤后21～23d愈合，C组于伤后24～26d愈合，A组较其它两组分别提前5～6d与8～9d愈合，且愈合质量好。（2）A组创面于移植15～17d，其肉芽组织生长旺盛，肉芽组织中Laminin、成纤维细胞和毛细血管含量丰富，可见胶原沉积。两对照组创面组织仍见许多炎细胞浸润，肉芽组织中Laminin、成纤维细胞和毛细血管含量少，胶原沉积不明显。结论PDGF-A基因修饰皮片移植对眼睑皮肤的创伤有较好的促愈作用，愈合质量较高。Objective To discuss PDGF-A gene modification in eyelid skin wound repair and the mechanism. Methods 6 minipigs were used in this study; 3 round full-thickness wounds （Ф3.67cm） were created on the dorsum of each side close to the vertebral column in each pig; in total, 36 wounds were created; 18 right side wounds were treated with skin sheet of PDGF-A gene modification as experimental group （group A）, 6 left side wounds were treated with human amniotic membrane （HAM） loaded withbonemarrow-derivedmesenchymalstemcells （bMSCs） and keratinocyte （group B） and 12 left side wounds were treated with HAM as control （group C）; the granulation tissue, reepithelization and wound area were observed after 1, 2, 3 and 4 weeks; immunohistochemistry was performed using Laminin as a marker for blood vessels, as well as the detection of collagen content. Results The healing time of group A was 15 to 17 days, that of group B was 21 to 23 days and that of group C was 24 to 26 days; the healing time of group A was 5 to 6 days faster than that of group B and 8 to 9 days faster than that of group C; 15 to 17 days after the graft, the granulation tissue, which contained a lot of Laminin, fibroblasts, capillaries and collagen, grew quite well in group A while many inflammatory cells were still seen in group B and group C, and the contents of Laminin, fibroblasts, capillaries and collagen in granulation tissue were smaller than those in group A. Conclusions The graft of skin sheet of PDGF-A gene modification is of good healing effect on eyelid skin wounds, the wounds heal with high quality.

关 键 词：PDGF-A基因修饰皮片 全层皮肤缺损创面 眼睑皮肤组织修复 

分 类 号：R779.6[医药卫生—眼科]