IgG4相关疾病与人类白细胞抗原-DR、DQ等位基因的相关性  

Correlation between HLA-DQ,DR Aleles and IgG4-related Diseases

作　　者：于立杰[1] 陈雨[2] 张盼盼[2] 张文[2] 

机构地区：[1]山东省聊城市东阿县人民医院风湿免疫科,山东聊城252200 [2]中国医学科学院北京协和医学院北京协和医院风湿免疫科风湿免疫病学教育部重点实验室,北京100730

出　　处：《中华临床免疫和变态反应杂志》2016年第4期346-350,共5页Chinese Journal of Allergy & Clinical Immunology

基　　金：国家自然科学基金(81373190,81571587)

摘　　要：目的探讨IgG4相关疾病(IgG4-related disease,IgG4-RD)与人类白细胞抗原(human leukocyte antigen,HLA)-DR、DQ等位基因的相关性。方法共纳入北京协和医院IgG4-RD队列研究患者91例,健康对照100例,采用序列特异性引物-聚合酶链反应(polymerase chain reaction-sequence specific primer,PCR-SSP)检测方法进行HLA-DR、HLA-DQ基因亚型分型。结果与健康对照组相比,IgG4-RD患者HLA-DRB1*03(13%vs.5%,P=0.047)表达率显著增高;HLA-DRB1*09表达率显著降低(16%vs.33%,P=0.009)。根据脏器受累不同,其基因表达率亦不相同。其中HLA-DRB1*03、HLA-DRB1*08在胰腺受累患者,HLA-DRB1*07、HLA-DQB1*02在颌下腺受累患者,HLA-DRB1*03、HLA-DQB1*02在泪腺受累患者,HLA-DRB1*04、HLA-DRB1*07、HLA-DQB1*02在腮腺受累患者,HLADRB1*07在泌尿系统受累患者,HLA-DRB1*03、HLA-DRB1*07、HLA-DQB1*02在鼻窦受累患者,HLA-DRB1*08在腹膜后受累患者,HLA-DRB1*04在肺部受累患者表达率显著升高。结论 IgG4-RD患者携带HLA-DRB1*03频率较高,该基因可能与疾病易感有关。不同脏器受累患者HLA-DR、HLA-DQ的基因亚型表达率不同。Objective To analyze the correlation between HLA-A DR, DQ alleles and IgG4-related diseases （IgG4-RD）. Methods HLA-DR, DQ alleles were tested in 91 patients with IgG4-RD and 100 normal controls by polymerase chain reaction-sequence specific primers （PCR-SSP）. Results The frequency of HLA-DRB1 ＊ 03 in IgG4-RD group was much higher than that in control group with significant statistical difference （ 13% vs. 5% , P =0. 047） ; The frequency of HLA-DRB1 ＊ 09 in IgG4-RD group was much lower than that in control group with significant statistical difference （ 16% vs. 33%, P = 0. 009 ）. In addition, the gene expression level also showed difference according to different organ involvement. The frequency of HLA-DRB1 ＊ 03 and HLA-DRB1 ＊ 08, HLA-DRBI ＊ 07 and HLA-DQB1 ＊ 02, I-ILA-DRB1 ＊ 03 and HLA-DQB1 ＊ 02, HLA-DRB1 ＊ 04, HLA-DRB1 ＊ 07 and HLA-DQB1 ＊ 02, HLA-DRB1 ＊ 07HLA-DRB1 ＊ 03, HLA-DRB1 ＊ 07 and HLA-DQB1 ＊ 02, HLA-DRB1 ＊ 08, as well as HLA-DRB1 ＊ 04 were higher in patients with of pancreatic gland, submandibular gland, lacriminal gland, parotid, urinary tract, sinus, retroperitoneal fibrosis and lung involvement, respectively. Conclusion Our results suggest that HLA-DRB1 ＊ 03 might be the susceptible gene in IgG4-RD. The gene expression also shows differ- ence according to different organ involvement.

关 键 词：IG G4相关疾病 人类白细胞抗原 易感基因 

分 类 号：R593.2[医药卫生—内科学]