KT对刺葡萄愈伤组织花青素含量的影响  

Effect of KT on anthocyanin content in callus of brier grape(Vitis davidii Foёx)

作　　者：赖呈纯[1] 黄贤贵[1] 黄金泽 潘红[1] 范丽华[1] 谢鸿根[1] 王琦[1] LAI Cheng-chun HUANG Xian-gui HUANG Jin-ze PAN Hong FAN Li-Hua XIE Hong-Gen WANG Qi(Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Sciences, Fujian Province 350003 Agricultural Economy and Science and Technology Information Research Institute, Fuiian Academ~ of Agricultural Sciences. Fuiian Provide)

机构地区：[1]福建省农业科学院农业工程技术研究所,350003 [2]福建省农业科学院农业经济与科技信息研究所

出　　处：《福建农业科技》2016年第9期1-3,共3页Fujian Agricultural Science and Technology

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1015-6);福建省自然科学基金项目(2016J01126);福建省种业创新与产业化工程项目(FJZZZY-1520);"十二五"农村领域国家科技计划项目(2013BAD01B05-7)

摘　　要：以长期保持的紫红色刺葡萄愈伤组织DLR细胞系为试验材料,研究不同KT浓度对其花青素和原花青素含量的影响,结果表明:KT可以有效调控刺葡萄愈伤组织花青素和原花青素的合成,其中以MS+2,4-D 1.0 mg/L+KT 0.05 mg/L培养基效果最佳,花青素和原花青素含量分别达到66.95μg/g(FW)和3947.50μg/g(FW),分别是对照的2.44倍和1.68倍。Using the long-term maintained and purplish red callus of brier grape as test material,the effect of different KT concentration on its anthocyanin and proanthocyanidinscontent was investigated in this paper.The result showed that the anthocyanin and proanthocyanidins synthesis of brier grape callus could be regulated effectively by KT.The optimal medium was MS ＋ 2,4-D 1.0 mg/L ＋ KT 0.05 mg/L,the anthocyanincontent and proanthocyanidinscontent were up to 66.95 μg/g（FW） and 3947.50 μg/g（FW）,and they were 2.44 times and 1.68 times as control respectively.

关 键 词：刺葡萄 6-糠氨基嘌呤(KT) 花青素 原花青素 愈伤组织 

分 类 号：S663.1[农业科学—果树学]