血清E-cadherin在甲状腺乳头状癌中的表达及意义  

Expression and significance of serum Ecadherin in patients with papillary thyroid carcinoma

作　　者：孙琳[1] 王冬梅[2] 张文静[2] 张静[2] 吴靖芳[2] 

机构地区：[1]河北北方学院人体解剖学教研室,河北张家口075000 [2]河北北方学院组织胚胎学教研室

出　　处：《临床耳鼻咽喉头颈外科杂志》2017年第2期123-126,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：河北省教育厅青年基金(No:QN2015204);河北省教育厅重点项目(No:ZD2016094);河北省卫生厅医学研究课题(No:20130035);河北省重大医学科研课题(No:ZD2013052);河北北方学院青年基金项目(No:Q2014020)

摘　　要：目的:通过对甲状腺乳头状癌、结节性甲状腺肿患者及正常人群血清E-cadherin浓度的测定,结合E-cadherin蛋白在乳头状癌、结节性甲状腺肿和癌旁正常组织中的检测,分析E-cadherin在甲状腺乳头状癌中的作用及意义。方法:采用ELISA法检测E-cadherin在86例甲状腺乳头状癌、16例结节性甲状腺肿患者和10例正常人血清中的含量,并分析其与甲状腺乳头状癌临床病理特征的关系;应用Western Blot法检测E-cadherin蛋白在甲状腺乳头状癌、结节性甲状腺肿和癌旁正常组织中的含量。结果:甲状腺乳头状癌患者血清E-cadherin的含量明显高于结节性甲状腺肿患者和正常人(P<0.05),结节性甲状腺肿和正常人血清中E-cadherin的含量无明显差异(P>0.05)。E-cadherin蛋白在乳头状癌组织中的含量明显低于结节性甲状腺肿、癌旁组织(P<0.05),结节性甲状腺肿、癌旁组织中含量无明显差异(P>0.05)。血清中E-cadherin的高水平表达与甲状腺乳头状癌的临床病理分期和淋巴结转移相关(P<0.05),而与患者的性别、年龄及肿瘤直径大小不相关(P>0.05)。结论:血清E-cadherin上调与甲状腺乳头状癌的临床病理分期和远处淋巴结转移相关。患者血清中E-cadherin含量的检测,在甲状腺乳头状癌诊疗及预后方面具有重要意义。Objective：Experiments testing the concentration of serum E-cadherin were conducted through the patients with papillary thyroid carcinoma（PTC）,nodular goiter and normal people.Combining the detection of Ecadherin protein in the tissues including papillary thyroid carcinoma,nodular goiter and normal thyroid tissues around adenoma,we analyze the effect and siginificance of E-cadherin in the papillary thyroid carcinoma.Method：The concentrations of E-cadherin in the serum of 86 cases of PTC patients,16 cases of NG patients and 10 normal adults were detected by enzyme linked immunosorbent assay（ELISA）method,and the relationships between the serum level and clinical pathology characteristics of thyroid papillary carcinoma were explored.Western blot were used to detect the content of E-cadherin protein in tissues including PTC,nodular goiter tissues and normal thyroid tissues around adenoma.Result：The results showed that content of serum E-cadherin in PTC were significantly higher than those in nodular goiter tissues and normal thyroid issues（P〈0.05）.There were no obviously differences between nodular goiter tissues and normal thyroid issues in the content of E-cadherin（P〉0.05）.The content of E-cadherin protein in the tissues of PTC was evidently lower than those in nodular goiter tissues and normal thyroid tissues around adenoma（P〈0.05）.There were no obviously differences between nodular goiter tissues and normal thyroid tissues around adenoma in the content of E-cadherin（P〉0.05）.The abnormal expression of serum E-cadherin was correlated with the lymph node metastasis and pathological stage of PTC（P〈0.05）.On the other hand,it is irrelevant to the gender、age and the tumor size of the patients（P〉0.05）.Conclusion：The up-regulation of serum E-cadherin is correlated with the lymph node metastasis and pathological stage of PTC.The detection of the serum E-cadherin content plays a vital role in the aspects of early-diagnosis and prognosis evaluation.

关 键 词：甲状腺乳头状癌 血清 E-钙黏连蛋白 

分 类 号：R736.1[医药卫生—肿瘤]