灯盏花素对动脉粥样硬化大鼠氧化应激和炎症反应的影响  

The Influences of Breviscapine on Oxidative Stress and Inflammation in Atherosclerosis Rats

作　　者：张玥[1] 朱晓冉[1] 蔡玥[1] 曹格溪 詹文红[2] 

机构地区：[1]河北省人民医院药学部,河北石家庄050051 [2]河北医科大学药学院,河北石家庄050017

出　　处：《贵州医科大学学报》2017年第1期71-75,共5页Journal of Guizhou Medical University

摘　　要：目的:研究灯盏花素(Bre)对动脉粥样硬化大鼠氧化应激和炎症反应的影响。方法:选取96只大鼠随机分为正常对照组,模型组,Bre 6、12、24 mg/(kg·d)组和辛伐他汀(SV)组(n=16),后5组采用高脂饲料喂养结合腹腔注射维生素D3的方法建立动脉粥样硬化大鼠模型,各治疗组造模过程同步灌胃给药,正常对照组给予正常饮食及腹腔注射等体积生理盐水;通过HE染色观察主动脉病理变化并进行病变分级;比色法测定血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)活性和丙二醛(MDA)、活性氧簇(ROS)含量;酶联免疫法测定血浆中C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)、白介素-10(IL-10)含量。结果:与模型组比较,Bre干预组大鼠主动脉病理改变呈不同程度改善,Bre24 mg/(kg·d)组病变分级显著降低(P<0.01),Bre 12、24 mg/(kg·d)组血清中SOD、CAT活性显著升高(P<0.05),MDA、ROS含量显著降低(P<0.05),血浆中CRP、TNF-α、IL-1β及IL-6含量显著降低(P<0.05),Bre24 mg/(kg·d)组GSH-Px活性显著升高(P<0.05),IL-10含量显著升高(P<0.01)。结论 :Bre可能通过抑制氧化应激和炎症反应而改善动脉粥样硬化程度。Objective： To investigate the inhibitory effects of breviscapine （Bre） on oxidative stress and inflammation in atherosclerosis rats. Methods： Eighty rat models were made by dirtying with high fat and injecting VD3 and was divided into 5 groups： model group, Bre 6, 12, 24 mg/（ kg· d） groups and 1.8 mg/（ kg· d） SV group （n = 16）, and the drugs were given by intragastric administration at the same time; setting normal control group （n = 16）. The histopathological changes of abdominal aorta was observed by HE staining and graded; the activity of antioxidases and the content of MDA, and ROS in serum were determined; the levels of CRP, TNF-α, IL-1β, IL-6, and IL-10 in the plasma were determined with enzyme linked immunosorbent assay. Results： Compared with model group, the histopathological changes of the rats in Bre treated groups were improved, especially in Bre 24 mg/（ kg · d） treated group （P 〈0.01）; in Bre 12, 24 mg/（kg· d） treated groups, the activity of SOD, CAT in serum was significantly increased （P 〈 0.05 ）, the contents of MDA, ROS in serum were significantly decreased （P 〈 0.05 ）, and the levels of CRP, TNF-α, IL-1β, IL-6 in plasma were signifi-cantly decreased （ P 〈 0. 05 ） ; in Bre 24 rag/（ kg· d） treated group, activity ot G^tt-Px in serum was significantly increased （ P 〈 0. 05 ） , and the level of IL-10 in plasma was significantly increased （P 〈 0. 01 ）. Conclusion： Bre might mitigate atherosclerosis through restrain oxidative stress and inflamma- tion reaction.

关 键 词：灯盏花素 动脉硬化 氧化性应激 C反应蛋白 肿瘤坏死因子-Α 白细胞介素 

分 类 号：R363[医药卫生—病理学]