正畸移动大鼠牙过程牙周组织中PLAP-1、BMP-2的表达及意义  

Expressions of PLAP-1 and BMP-2 in Periodontal Tissue of Rat during Orthodontic Tooth Movement

作　　者：周骢 伍宝琴 朱中焰 谭红 

机构地区：[1]西南医科大学附属口腔医院口腔内科,四川泸州646000

出　　处：《口腔医学研究》2017年第1期33-37,共5页Journal of Oral Science Research

基　　金：泸州市科技局资助项目(编号:20140101)

摘　　要：目的:研究正畸移动大鼠上后牙过程中,PLAP-1、BMP-2在牙周膜的分布和表达。方法:选择雄性SD大鼠36只,按1d、3d、5d、7d、14d、21d分为6组,均用50g移动左上第一磨牙,右上第一磨牙为对照。在相应时期处死大鼠后测量牙移动距离,采用HE染色及免疫组织化学SP法,检测不同时期牙周组织的形态变化;PLAP-1、BMP-2在牙周膜组织中的表达及研究两者可能存在的关系。结果:不同时期牙移动的距离不同,差别显著有统计学意义(P<0.05)。HE染色显示对照组牙周组织变化不明显;实验组张力区有成骨细胞生成,压力区破骨细胞生成,随着时间的延长最后两区形态都基本趋一致。免疫组化法示PLAP-1在牙周膜的表达总是张力区高于压力区,在第3天张力区表达最强,除21d外,其余各组间差异显著有统计学意义(P<0.05)。BMP-2在第5天张力侧表达最强,在3d、5d、7d的表达差异显著有统计学意义(P<0.05)。结论:PLAP-1,BMP-2在正畸力作用下参与牙周膜组织改建的表达最高值时期不同。Objective：To examine the expression of periodontal ligament-associated protein-1（PLAP-1）and bone morphogenetic protein-2（BMP-2）in the periodontal tissue of rats during the orthodontic tooth movement.Methods：A total of 36 male SD rats were allocated into 6groups evenly,d1,d3,d5,d7,d14,d21.The power of50 g was applied on the left maxillary first molar,and the distance of orthodontic tooth movement was measured on the different time.HE staining and immunohistochemical sp method were used to detect the morphological changes,the expression of PLAP-1and BMP-2of the periodontal tissue at different time points.Results：The distances of orthodontic tooth movement were different significantly among 6groups.HE results showed no significant changes in the periodontal tissue in control groups in different time;while in the experimental group,osteoblasts were regenerated in the tension side and osteoclasts regenerated in the compressive side of periodontal tissue.The expression of PLAP-1on both sides reached the peak on the 3din the experimental group,and higher on the tensional side than the compressive side,there existed significant difference among all the groups except for the 21 dgroup.The expression of BMP-2on both sides reached the peak on the 5din the experimental group,and higher on the tensional side than the compressive side.Conclusion：The expression of PLAP-1and BMP-2reached the peak at different time points.

关 键 词：PLAP-1 BMP-2 正畸牙移动 免疫组化 牙周膜 

分 类 号：R783.5[医药卫生—口腔医学]