粪便microRNA-221对早期结直肠癌检出的意义  

The Importance of micro RNA-221 in Stool for Diagnosis Early Colorectal Cancer

作　　者：胡晶晶[1] 曾理[1] 李开学[1] 刘若丹[1] 张永锋[1] 熊鹰[1] HU Jing-jing ZENG Li LI Kai-xue LIU Ruo-dan ZHANG Yong-feng XIONG Ying(Shenzhen Second People＇s Hospital, Guangdong Shenzhen 518029)

机构地区：[1]深圳市第二人民医院,广东深圳518029

出　　处：《深圳中西医结合杂志》2016年第22期25-27,F0003,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市科技创新委员会资助项目资助课题(JCYJ20140414170821182)

摘　　要：目的:探讨micro RNA-221在早期结肠癌患者粪便中的表达情况,并研究其作为早期结直肠癌以及癌前病变分子诊断标志物的可行性。方法:采用实时荧光定量聚合酶链反应检测245例大肠病变患者的粪便及30例正常人群粪便中micro RNA-221的水平,分析micro RNA-221与不同临床病理分级的关系。结果:结肠病变的患者粪便中micro RNA-221水平随着病理分级增加而增高,差异具有统计学意义(P<0.001)。进展型腺瘤和早期结直肠癌组织中micro RNA-221水平与对照组相比明显升高,差异有统计学意义(P<0.001)。结论:早期结直肠癌粪便中micro RNA-221的表达上调,可能参与了结直肠癌的发生及发展,粪便micro RNA-221有可能成为筛查早期结直肠癌非侵入性的标志物。Objective The detection of microRNA （microRNANA） dysregulation in stool is a novel approach for the diagnosis of early colorectal cancer. The aim of this study is to investigate the use of microRNA-221 in stool samples as non-invasive biomarker for early colorectal cancer diagnosis. Methods The levels ofmicroRNA-221 in 245 stool samples with colorectal lesions were measured by real-time PCR. The relationship between the expression ofmicroRNA-221 and clinicopathological features was analyzed. Results In stool samples, the level of microRNA-221 showed a significant increasing trend with the increase of pathological grade（P 〈 0.001）.The level of microRNA-221 in tissue of colorectal lesions was statistically higher than in the controls（3.02 ± 0.68 VS 1.10 ±0.19, P 〈 0.001 ）. Conclusion The over-expressd microRNA-221 might play a role in the development and progression of colorectal carcinoma. Stool-based microRNA-221 can be used as a non-invasive biomarker for prognosis of early colorectal cancer.

关 键 词：结直肠癌 microRNA-221 生物标志物 

分 类 号：R735.3[医药卫生—肿瘤]