亚抑菌浓度头孢他啶对大肠埃希菌胞外多糖和pga基因的影响  

Effect of Sub-minimal Inhibitory Concentrations of Ceftazidime on Extracellular Polymeric Substances and pga Gene of Escherichia coli

作　　者：孙凤军[1] 冯伟[1] 刘耀[1] 枉前[2] 夏培元[1] SUN Feng-jun FENG Wei LIU Yao WANG Qian XIA Pei-yuan(Department of Pharmacy,Southwest Hospital, Third Military Medical University, Chongqing 400038,China Department of Pharmacy, Xinqiao Hospital, Third Military Medical University,Chongqing 400037,China)

机构地区：[1]第三军医大学西南医院药剂科,重庆400038 [2]第三军医大学新桥医院药剂科,重庆400037

出　　处：《解放军药学学报》2016年第6期494-496,501,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金项目;No.81373451

摘　　要：目的明确亚抑菌浓度头孢他啶在大肠埃希菌生物膜抑制过程中对胞外多糖和pga基因的影响。方法采用标准琼脂平板倍比稀释法检测细菌最低抑菌浓度,采用96孔板结晶紫染色法检测生物膜形成能力,采用苯酚-硫酸法测定胞外多糖含量,pgaABCD基因表达采用RT-PCR扩增。结果大肠埃希菌对氨苄西林和氟喹诺酮类耐药率较高,而对阿米卡星和碳青霉烯类全部敏感。亚抑菌浓度头孢他啶对大肠埃希菌生物膜形成、胞外多糖产生能力和pgaABCD基因表达均有显著抑制作用,但其降低程度不完全一致。结论亚抑菌浓度头孢他啶在抑制生物膜形成过程中能降低大肠埃希菌胞外多糖产生和pga基因表达,但其与生物膜降低程度存在差异性。Objective To analyze the effect of sub-minimal inhibitory concentrations（sub-MIC）of ceftazidime on extracellular polymeric substances（EPS）and pgagene of Escherichia coli in the process of biofilm inhibition.Methods The minimal inhibitory concentration was detected by the agar double dilution method.The biofilm formation was assayed using the 96-well crystal violet staining method.The content of EPS was determined by phenol-sulfuric acid method.The expression of pgaABCD gene was analyzed by Real-Time PCR amplification.Results E.coli isolates were highly resistant to ampicillin and fluoroquinolones,but were all sensitive to amikacin and carbapenems.Sub-MIC ceftazidime could inhibit the biofilm formation,EPS production and pgaABCD gene expression of E.coli,but the reduction rates were not fully consistent.Conclusion Sub-MIC ceftazidime can decrease EPS production and pgaABCD gene expression of E.coli during the inhibition of biofilm formation,but the reduction levels are different from those of biofilm formation.

关 键 词：大肠埃希菌 头孢他啶 生物膜 亚抑菌浓度 胞外多糖 pga基因 

分 类 号：R961[医药卫生—药理学]