利用装载内皮细胞生长因子的丝素纳米球制备具有生物活性的电纺支架材料  

The Fabrication of Bioactive Electrospun Scaffolds Using Silk Fibroin Nanoparticles Loading with VEGF

作　　者：王恺[1] 马绍扬 张钧[1] Wang Kai Ma Shaoyang Zhang Jun(College of Life Sciences, Nankai University, Tianjin 300071, China)

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2016年第6期80-85,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学青年基金(31401150;81471799;81401534);天津市应用基础与前沿技术研究计划青年项目(14JCQNJC13900)

摘　　要：蚕丝纤维作为一种天然材料,是最早被利用的生物材料之一,其中含有的丝素蛋白使其具有优良的力学性能,组织相容性及可降解性,在生物医学领域得到广泛关注.将丝素蛋白从蚕丝中提取出来,制成纳米球,使其负载内皮细胞生长因子(VEGF)并混入聚己内酯(PCL)纺丝液中,通过静电纺丝技术制备得到纤维内含有丝素纳米球的PCL电纺膜.体外释放表明丝素纳米球及含丝素纳米球的电纺纤维膜都有很好的缓释效果;生物体内评价结果表明负载有VEGF的丝素纳米球混合的PCL电纺支架促进了体内的细胞浸润和周围血管化.Silk fiber is a kind of natural materials,and it is one of the earliest biological materials.Silk fiborin is the main component of the silk fiber,and it contribute to the mechanical property,tissue compatibility and degradable property.In this paper,silk fiborin was extracted from the silk fiber and made into nanoparticles,then loaded with vascular endothelial growth factor and mixed into the PCL electrostatic spinning solution.The nanofiber film of PCL combined with silk fiborin can be obtained ith electrospinnmg technology.The in vitro release of VEGF indicate that the silk fiborin nanoparticles and films with silk fiborin nanoparticles show sustained-release effect;the evaluation of in vivo study indicate that the PCL scaffold combined with VEGF-silk fiborin nanoparticles promote the cell infiltration and vascularization.

关 键 词：组织工程 静电纺丝 丝素蛋白 丝素纳米球 VEGF 

分 类 号：Q819[生物学—生物工程]