基于间苯二甲醛构建的IPEI在滑膜细胞中的转染与毒性研究  

Transfection and Cytotoxicity Studies of Novel PEI Derivative IPEI in Synovial Cells

作　　者：马柳青[1] 车俊怡 原明璐 欧阳元明[2] 袁伟恩[1] MA Liu-qing CHE Jan-yi YUAN Ming-lu OUYANG Yuan-ming YUAN Wei-en(School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People＇s Hospital, Shanghai, 200233, China)

机构地区：[1]上海交通大学药学院,上海200240 [2]上海交通大学附属第六人民医院,上海200233

出　　处：《现代生物医学进展》2016年第35期6801-6803,6811,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81373366);上海市自然科学基金项目(15ZR1432500);上海交通大学医工结合项目(YG2013MS52)

摘　　要：目的:研究以间苯二甲醛(Isophthalaldehyde)作为连接剂构建的新型聚乙烯亚胺(Polyethyleneimine,PEI)衍生物IPEI为非病毒基因载体在治疗类风湿性关节炎(RA)中的应用,主要对阳离子聚合物IPEI在SD大鼠滑膜细胞中进行体外生物学评价。方法:IPEI以不同质量比包裹绿色荧光蛋白(GFP)质粒和白细胞介素-1受体拮抗剂(IL-1Ra)质粒,研究复合物在滑膜细胞中的转染活性,同时用MTT方法研究聚合物对滑膜细胞的细胞毒性。结果:复合物在质量比为2到10范围内,转染效果比较理想,具有高效输送质粒的能力;同时IPEI的细胞毒性也很小,细胞存活率明显高于商业化转染试剂PEI 25 KDa对照组。结论:阳离子聚合物IPEI具有高转染效率和低毒性的特点,从体内水平上证实了IPEI是一种良好的治疗类风湿性关节炎的基因输送载体。Objective： Novel PEI derivative IPEI is designed and synthesized by using isophthalaldehyde as linkers. This study is to investigate the transfection efficiency and cell cytotoxicity of IPEI, which can be applied as non-viral carriers in rheumatoid arthritis gene therapy. Methods： IPEI was packed with GFP and IL-1Ra pDNA at different mass ratios, transfection efficiency of this polyplex in synovial cells of SD rats was investigated. Cytotoxicity of 1PEI was detected by MTT method. Results： IPEI could effectively deliver pDNA into synovial cells to express related protein, especially when mass ratio was 2 to 10;IPEI had lower cytotoxicity than commercial transfection agent PE125 KDa at the same concentration and the cell viability was very high. Conclusions： 1PEI had high transfection efficiency and low cytotoxicity, indicating that it had a bright application prospect in rheumatoid arthritis gene therapy as non-viral carriers.

关 键 词：类风湿性关节炎 基因治疗 IL-1RA 体外转染 细胞毒性 

分 类 号：R-33[医药卫生] R593.22