DMP1在去势大鼠颌骨中的表达  

The Expression of DMP1 in the Mandible of Ovariectomized Rat

作　　者：吴迪[1] 张斌[1] 李莹[1] 牟海滨[1] 高丽[1] 赵芳[1] 刘明月[1] WU Di ZHANG Bin LI Ying MU Hai-bin GAO Li ZHAO Fang LIU Ming-yue(The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongfiang, 150086, China)

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2016年第35期6808-6811,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81570951)

摘　　要：目的:观察卵巢去势骨质疏松大鼠上颌牙牙本质基质蛋白1(DMP1)的表达变化。方法:选择三月龄健康雌性wistar大鼠40只,在SPF级动物房内适应性饲养一周后,随机分为二组:Sham组(假手术组,仅去除和双侧卵巢等大的脂肪组织)20只;Ovx组(手术组,去除双侧卵巢)20只。实验周期为12 w。术后4 w,8 w,12 w取材,根据实验需要留取腰椎和下颌骨。腰椎用于检测骨密度,下颌骨储存于液氮用于逆转录-聚合酶链反应(RT-PCR)检测不同组别不同时间DMP1的基因水平的表达。结果:术后12 w时,OVX组腰椎骨密度与Sham组相比明显下降,差异有统计学意义(P<0.05)。术后4 w及8 w时,两组之间DMP1的m RNA表达比较无统计学差异(P>0.05),术后12 w时,Ovx组DMP1的m RNA表达显著高于Sham组,差别有统计学意义(p<0.05)。结论:卵巢去势骨质疏松大鼠下颌骨中DMP1 m RNA的表达明显下降,DMP1可能在雌激素缺乏所致骨质疏松的发生过程中具有一定的作用。Objective： To observe the expression of Dentin Matrix Protein 1 （DMP 1） in the mandible of ovariectomized rat Methods： 40 healthy female wistar rats （three months＇ old） were selected and randomly divided into two groups（20 rats each group） after 1 week of adaptive breeding in the SPF animals house Sham group （sham operation group, only bilateral ovarian big fat tissue was remove）; Ovx group （surgery, bilateral ovaries were removed）. The experimental period was 12 weeks. According to the need, lumbar vertebrae and bi- lateral mandible were obtained at 4, 8, 12 weeks postoperation. Lumbar spines were used to detect the bone mineral density. The mandible was stored in liquid nitrogen for testing the expression level of DMP1 in different groups at different time by using reverse tran- scription-polymerase chain reaction （RT-PCR）. Results： No significant difference was found in the expression of DMP-1 between the Sham group and Ovx group at 4 w, 8 w （P〉0.05） postoperation. However, the expression of DMP-1 was significant difference between Sham group and Ovx group at 12 w postoperation （P〈0.05）. Conclusions： The expression ofDMP1 mRNA was significant decreased in mandible of ovary castration osteoporosis rats, which might play an important role in the development of osteoporosis aroused by a lack of estrogen.

关 键 词：牙本质基质蛋白 骨质疏松 细胞外基质 卵巢去势 

分 类 号：R-33[医药卫生] R782