K-ras基因突变与结直肠癌临床病理特征的相关性研究  

Correlative Research of K-ras Gene Mutation with the Clinical Pathological Features of Colorectal Cancer

作　　者：祝继原[1] 吴鹤[1] 张振[1] 王梦迪[1] 张世超[1] 鹿芃恬 戚基萍[1] ZHU Ji-yuan WU He ZHANG ghen WANG Meng-di ZHANG Shi-chao LU Peng-tian QI Ji-ping(Department of Pathology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China)

机构地区：[1]哈尔滨医科大学附属第一医院病理科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2016年第35期6848-6851,共4页Progress in Modern Biomedicine

基　　金：黑龙江省应用技术研究与开发计划项目(GA14C101-03)

摘　　要：目的:探讨结直肠癌患者K-ras基因突变特点,并分析K-ras基因突变与结肠癌临床病理特征的相关性及其临床意义。方法:收集哈尔滨医科大学附属第一医院收治的500例结直肠癌患者的手术石蜡标本,采用突变扩增阻滞系统技术(amplification refractory mutation system,ARMS)检测K-ras基因第二外显子第12、13密码子的七种热点突变情况,分析其与结肠癌患者临床病理特征的相关性。结果:ARMS法检出K-ras基因突变213例,突变率为42.6%。K-ras基因突变与患者的年龄、性别、肿瘤发生的部位、肿瘤分化程度及有无淋巴结转移均无相关性(P>0.05),而与肿瘤的组织学类型具有相关性(P<0.05)。结论:(1)结直肠癌患者K-ras基因突变率较高,其中GLY12ASP位点突变率最高,GLY12ARG为稀有突变位点。(2)K-ras基因突变与结肠癌的组织学类型相关,可能用于结直肠癌患者治疗方案的选择。Objective： To investigate the characteristics of K-ras gene mutation in colorectal cancer patients, and analyze the correlation of K-ras gene mutation with clinicopathological features of colorectal cancer. Methods： 500 cases of specimens from patients with colorectal cancer surgery in the First Affiliated Hospital of Harbin Medical University were collected, amplification refractory mutation system block （amplification refractory mutation system technology arms） detection was used to detect K-ras gene second significantly sub 12 and 13 codon seven hot mutation, its correlation with the clinical and pathological features of colon cancer patients were analyzed. Results： 213 cases of K-ras gene mutation were detected by ARMS method, and the mutation rate was 42.6%. K-ras gene mutations were not correlated with the age, sex, tumor location, tumor differentiation degree and lymph node metastasis （P〉0.05）, but were correlated with the histological type of tumor （P〈0.05）. Conclusion： （1）The mutation rate of K-ras gene in colorectal cancer patients was high, among which the mutation rate of GLY12ASP was the highest, and GLY12ARG was the rare mutation site. （2） K-ras gene mutation was associated with the histological type of colon cancer, and might be used in the treatment of colorectal cancer patients.

关 键 词：K-RAS基因 结直肠癌 突变扩增阻滞系统技术 

分 类 号：R735.3[医药卫生—肿瘤]