基于凝胶渗透色谱-激光光散射联用技术的聚天冬氨酸分子量测定方法的建立  

Establishment of determination method for the molecular weight of polyaspartic acid by gel permeation chromatography-multi-angle light scattering

作　　者：刘蕊[1] 马博凯[1] 勾新磊[1] 周明强[1,2] LIU Rui MA Bo-Kai GOU Xin-Lei ZHOU Ming-Qiang(Beijing Centre for Physical and Chemical Analysis, Beijing Key Laboratory of Organic Materials Testing Technology ＆ Quality Evaluation, Beijing 100089, China Beijing Academy of Science and Technology Key Laboratory of Analysts and Testing Technology, Beijing 100089, China)

机构地区：[1]北京市理化分析测试中心有机材料检测技术与质量评价北京市重点实验室,北京100089 [2]北京市科学技术研究院分析测试技术重点实验室,北京100089

出　　处：《食品安全质量检测学报》2016年第11期4318-4322,共5页Journal of Food Safety and Quality

摘　　要：目的建立利用凝胶渗透色谱-激光光散射联用技术(gel permeation chromatography-multi-angle light scattering,GPC-MALS)对聚天冬氨酸分子量及分子量分布进行测定的方法。方法以市售聚天冬氨酸产品为样品,研究凝胶色谱柱的类型和样品浓度对检测结果的影响,利用GPC-MALS同时测定聚天冬氨酸的数均分子量、重均分子量和分子量分布等参数。结果采用Shodex Protein KW-803凝胶色谱柱,样品浓度为18.5 mg/m L时,可以得到较好的实验结果,聚天冬氨酸分子量和分子量分布测定的相对标准偏差分别为1.8%和0.7%。结论该方法操作简单,精密度良好,适用于聚天冬氨酸分子量及分子量分布的测定。Objective To establish a method for the determination of molecular weight and molecular weight distribution of polyaspartic acid （PASP） by gel permeation chromatography-multi-angle light scattering （GPC-MALS）. Methods Influences of the gel column type and concentration of sample on the detection results were studied taking commercially available polyaspartic acid products as samples. The number average molecular weight, weight average molecular weight and molecular weight distribution were simultaneously determined by GPC-MALS. Results The better results were obtained when the sample concentration was 18.5 mg/mL using the Shodex Protein KW-803 gel column. The relative standard deviations of molecular weight and molecular weight distribution were 1.8%and 0.7%respectively. Conclusion This method is simple and accurate, which can be used for the determination of the molecular weight and molecular weight distribution analysis of PASP.

关 键 词：聚天冬氨酸 分子量及分子量分布 凝胶渗透色谱-激光光散射联用技术 

分 类 号：O631.61[理学—高分子化学]