MiR-98靶向MTDH调控鼻咽癌恶性进展的实验研究  

MiR-98 regulates the malignant progression of nasopharyngeal carcinoma via targeting MTDH

作　　者：佘笠 谭浩蕾[1,2] 朱刚才[3] 魏明[1] 陈昌瀚[1] 皮雷鸣[1] 刘观成[1] 张叠阔 黄东海[1] 张欣[1] 刘勇[1] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉头颈外科耳鼻咽喉重大疾病研究湖南省重点实验室,湖南长沙410008 [2]中南大学湘雅医学院湖南省肿瘤医院头颈外科,湖南长沙410013 [3]中南大学湘雅二医院耳鼻咽喉头颈外科,湖南长沙410011

出　　处：《中国耳鼻咽喉颅底外科杂志》2016年第6期455-461,共7页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：国家自然科学基金(81272974;81372906;81472696;81202128);湖南省自然科学基金(2015JJ3137);高等学校博士学科点科研基金课题(20120162120049);中南大学自由探索计划青年教师助推基金(2012QNZT099)

摘　　要：目的探讨miR-98对鼻咽癌细胞体外增殖和侵袭能力的影响及其可能机制。方法利用脂质体介导的miR-98 mimic(及阴性对照)转染鼻咽癌CNE-1和CNE-2细胞,CCK-8检测其体外增殖能力的改变,划痕愈合及Transwell侵袭小室实验检测鼻咽癌细胞的体外迁移及侵袭潜能变化,生物信息学软件预测miR-98可能的靶基因,并经双荧光素酶及Western blotting验证靶基因。结果 MiR-98过表达能抑制鼻咽癌CNE-1和CNE-2细胞的体外生长增殖、迁移及侵袭能力;miR-98对MTDH具有直接靶向调控作用。结论本研究表明miR-98能够靶向MTDH调控鼻咽癌细胞株的体外生长增殖及侵袭能力。Objective To investigate the effect and mechanism of miR-98 on the proliferation and invasion in nasopharyngeal carcinoma （NPC） in vitro. Methods Liposome-mediated MiR-98 mimic and negative control were applied to transfect CNE-1 and CNE-2 cells. CCK-8 assay was used to examine the proliferation of the cells in vitro. Alterations of migration and invasion were assayed by scratches and transwell chambers. Target genes of miR-98 were predicted by online target gene prediction database. Dual luciferase reporter assay and western blotting were used to validate their binding. Results MiR-98 mimic successfully decreased capacities of growth, proliferation, migration and invasion in both CNE-1 and CNE-2 cell lines. Meanwhile, MTDH was a direct target gene of miR-98. Conclusion MiR-98 inhibits the growth, proliferation, migration and invasion of NPC cell lines by targeting MTDH.

关 键 词：鼻咽癌 MiR-98 MTDH 增殖 侵袭 

分 类 号：R739.63[医药卫生—肿瘤]