羟基红花黄色素A抑制H22小鼠肝癌移植瘤血管生成及减少MMP-3的表达  

HSYA inhibits angiogenesis of H22 transplanted tumor tissue and down-regulation of MMP-3 expression in mice

作　　者：张静宇[1] 杨芳芳[1] 李京敏[1] 刘春晓[1] 白咸勇[1] 

机构地区：[1]滨州医学院基础医学院人体解剖与组织胚胎学教研室,山东烟台264000

出　　处：《基础医学与临床》2017年第1期71-75,共5页Basic and Clinical Medicine

基　　金：滨州医学院科技重点计划(2015WS0500;2014WS0480)

摘　　要：目的研究红花组分羟基红花黄色素A(HSYA)对H22小鼠肝癌移植瘤组织新血管生成的抑制作用及对基质金属蛋白酶-3(MMP-3)的影响。方法建立H22小鼠肝癌移植瘤模型,第2天将小鼠随机分为对照组、索拉非尼组及HSYA组(1.125和2.25 mg/kg),HE观察肝癌移植瘤组织病理;用免疫组织化学法检测肿瘤组织中CD34的表达,计数微血管密度(MVD);免疫组化和Western blot分别检测瘤组织中MMP-3的表达。结果与对照组相比,HSYA组(1.125和2.25 mg/kg)的MVD显著降低(P<0.01),MMP-3蛋白在移植瘤组织中的表达明显减少(P<0.01),以HSYA组(2.25 mg/kg)效果最为显著,但不及索拉非尼组。结论 HSYA在一定浓度范围内抑制H22小鼠肝癌移植瘤血管生成,其作用可能与降低基质金属蛋白酶-3的蛋白表达有关。Objective To investigate the inhibitory effect of hydroxy safflor yellow A （HSYA） on angiogenesis of H22 tumor-bearing mice and it＇s effects on the protein expression of MMP-3. Methods After establishing the hepatoma model for 24 h, the mice were randomly divided into control group, sorafenib group and HSYA group, the dose HSYA group received intraperitoneal injection at different dosages （ 1. 125 and 2. 25 mg/kg）. The pathological changes were examined with HE staining, immunohistochemical staining and Western blot were applied to measure the expression of angiogenesis related factor（ MMP-3 ） and we also detected the microvessel density with CD34. Results Compared with control group, the tumor cells proliferation and the new angiogenesis in HSYA group were suppressed. The expression of MMP-3 in HSYA group was significant reduced. Especially the dose of 2.25 mg/kg HSYA group（P 〈0. 01 ）, and tumor MVD-CD34 was also significantly reduced（ P 〈 0. 01 ）. But the effect is not better than sorafenib group. Conclusions HSYA may inhibit angiogenesis of tumor tissue in a certain concentration range and the anti-angiogenesis effect of HSYA may be related to inhibition of the protein expression of matrix metalloproteinase- 3.

关 键 词：羟基红花黄色素A 基质金属蛋白酶-3 肝细胞癌 肿瘤血管生成 

分 类 号：R735.7[医药卫生—肿瘤] R33[医药卫生—临床医学]