光照和pH对药用真菌二型附毛孔菌液体培养过程中抗氧化活性的影响  

Effects of light and pH on the antioxidant activities of medicinal fungus Trichaptum biforme during liquid cultivation

作　　者：杨晓坡[1,2] 郑飞[3] 刘铁志[1] 孟歌[3] 司静[3] 

机构地区：[1]赤峰学院生命科学学院,内蒙古赤峰024000 [2]山西大学科学技术哲学研究中心,山西太原030006 [3]北京林业大学微生物研究所,北京100083

出　　处：《菌物学报》2017年第1期71-82,共12页Mycosystema

基　　金：中央高校基本科研业务费专项资金(2016ZCQ04)~~

摘　　要：本文以多酚含量、总抗氧化能力(total antioxidant capacity,T-AOC)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力和超氧化物歧化酶(superoxide dismutase,SOD)活性为测定指标,研究了不同光照和pH条件对药用真菌二型附毛孔菌Trichaptum biforme液体培养过程中抗氧化活性的影响。结果显示,二型附毛孔菌在液体培养过程中具有抗氧化活性。一定的光照可提高其分泌次级代谢产物的能力及抗氧化活性,14d光照条件下,多酚含量和T-AOC分别在第12天和第10天达到峰值,为1.85mg/m L和3.99U/m L;1d黑暗、1d光照交替的光照条件下,DPPH自由基清除率和SOD活性均于第2天达到最大值,分别为87.44%和0.63U/m L。不同pH条件对二型附毛孔菌液体培养过程中的抗氧化活性影响更大。当pH为9.0时,二型附毛孔菌在液体培养过程中分泌的多酚含量、T-AOC、SOD活性和DPPH自由基清除能力均最高,分别于第2天、第10天、第2天和第2天达到2.92mg/m L、5.22U/m L、0.70U/m L和95.07%。其次是pH 3.0,最后是pH 5.0和7.0,说明偏碱或偏酸条件更能刺激药用真菌二型附毛孔菌通过分泌大量次级代谢产物以抵抗来自微环境的氧化压力。Based on the tested indicators of polyphenol contents, total antioxidant capacity（T-AOC）, 1,1-diphenyl-2-picrylhydrazyl（DPPH） radicals scavenging activities, and superoxide dismutase（SOD） activities, effects of different light and pH conditions on the antioxidant activities of medicinal fungus Trichaptum biforme during liquid cultivation were explored. The results demonstrated that T. biforme had antioxidant activities during submerged culture. Light treatment to a certain extent could enhance secretion capacity of secondary metabolites and antioxidant activities of the fungus. After being illuminated for 14 d, the strain was shown to exert the highest capacities for secreting polyphenol and T-AOC, with the corresponding yields of 1.85mg/m L and 3.99U/m L on the 12 th and 10 th days. Higher DPPH radical scavenging efficiency and SOD activities were observed under alternation condition of dark for 1d and light for 1d, reaching their peaks of 87.44% and 0.63U/m L on the 2nd day. Effects of different pH conditions on the antioxidant activities of T. biforme during liquid cultivation were greater. When the pH was 9.0, the fungus exhibited better capacities of secreting polyphenol, T-AOC, and SOD, and performed remarkable scavenging activities against DPPH radicals, with respective values of 2.92mg/m L, 5.22U/m L, 0.70U/m L, and 95.07% on the 2nd, 10 th, 2nd, and 2nd days. T. biforme showed the highest antioxidant activities at pH 9.0, and secondly by pH 3.0, 5.0, and 7.0, suggesting that relatively alkali or acidic conditions could stimulate the medicinal fungus T. biforme to secret more secondary metabolites to resist the oxidative stress from microenvironment.

关 键 词：二型附毛孔菌 药用真菌 培养条件 抗氧化活性 

分 类 号：S567.39[农业科学—中草药栽培]