药用真菌桑黄液体培养过程中的抗氧化活性研究  

Antioxidant activities of medicinal fungus Sanghuangporus sanghuang during liquid cultivation

作　　者：郑飞[1] 孟歌[1] 安琪[1] 田雪梅[2] 司静[1] 

机构地区：[1]北京林业大学微生物研究所,北京100083 [2]青岛农业大学山东省应用真菌重点实验室,山东青岛266109

出　　处：《菌物学报》2017年第1期98-111,共14页Mycosystema

基　　金：中央高校基本科研业务费专项资金(BLX2014-36);国家自然科学基金(31301827)~~

摘　　要：桑黄Sanghuangporus sanghuang是一种珍稀的药用真菌,具有较大的应用潜力。本研究主要以菌丝体生物量、多酚含量、黄酮含量、抗坏血酸(Ascorbic acid,AA)含量、总抗氧化能力(Total antioxidant capacity,T-AOC)、抑制羟自由基能力(Restraining ability to hydroxyl free radicals,RAHFR)、超氧阴离子清除能力、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2’-连氮-双(3-乙基苯并噻唑-6-磺酸)[2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid),ABTS]自由基清除能力、铁离子还原能力(Ferric reducing antioxidant power,FRAP)和亚铁离子螯合能力为测定指标,对桑黄液体培养过程中的抗氧化活性进行了评价。结果显示,菌丝体生物量在2–10d内增长迅速,第10天达到最大值,在此过程中AA含量和T-AOC也出现了峰值,且多酚含量、黄酮含量和FRAP均呈上升趋势,说明菌株的抗氧化活性与其自身的生长状况、次级代谢产物分泌及还原能力等密切相关。此外,该菌株对羟自由基、超氧阴离子、DPPH自由基和ABTS自由基的清除能力也较强,于第12天、第10天、第2天和第14天分别达到73.06U/m L、46.78%、86.47%和94.16%,显示了较强的抗氧化活性。较高的亚铁离子螯合能力也说明桑黄在氧化胁迫下可启动自身的抗氧化系统以阻断自由基链反应。研究结果为更好地研究、开发和利用药用真菌桑黄提供了基础资料。As a valuable medicinal fungus, Sanghuangporus sanghuang has great potentials for various applications. Based on the tested indicators of mycelial biomass, polyphenol content, flavonoid content, AA content, T-AOC, RAHFR, superoxide radical scavenging ability, DPPH radical scavenging ability, ABTS radical scavenging ability, FRAP, and ferrous ion chelating activity, the antioxidant activities of S. sanghuang were evaluated during liquid cultivation. The results showed that the mycelial biomass increased dramatically in two to eight days, reaching its maximum at the 10 th day. During liquid cultivation, the increased peaks of AA content and T-AOC were observed, and the upward trends of polyphenol content, flavonoid content, and FRAP were found, suggesting that antioxidant capacity of the fungus was dependent on mycelial growth, secondary metabolites secretion, and reducing power. Additionally, the fungus exhibited remarkable scavenging activities against hydroxyl free radicals, superoxide radicals, DPPH radicals, and ABTS radicals, with respective values of 73.06U/m L, 46.78%, 86.47%, and 94.16% on the 12 th, 10 th, 2nd, and 14 th days. Higher ferrous ion chelating activity also indicated that S. sanghuang could activate its antioxidant system by blocking the free radical chain reaction, in order to protect itself from oxidative stress. The results facilitate further studies, development, and utilization of the medicinal fungus S. sanghuang.

关 键 词：药用真菌 桑黄 菌丝体生物量 抗氧化活性 

分 类 号：S567.39[农业科学—中草药栽培]