线粒体转录终止因子蛋白家族的表达以及在MPTP/MPP^+诱导下的表达变化  

Expression and alteration induced by MPTP/MPP^+ of mitochondrial transcription termination factors family

作　　者：张林冰 韩艳艳[1] 仇石 许珊[1] 左伋[1] 刘雯[1] 

机构地区：[1]复旦大学上海医学院细胞与遗传医学系,上海200032

出　　处：《解剖学报》2017年第1期19-24,共6页Acta Anatomica Sinica

基　　金：上海市科委基础研究重大重点项目(13JC1401100;13JC1401101)

摘　　要：目的探讨线粒体转录终止因子(MTERFs)蛋白家族在组织和细胞中的表达以及在神经毒素MPTP/MPP+诱导下的表达变化。方法取正常C57BL小黑鼠的脑、肝、肾组织,以及人神经母细胞瘤细胞SH-SY5Y、人心肌细胞HCM、人肝细胞L-02,检测MTERFs的蛋白表达情况;通过MPTP/MPP+构建帕金森病(PD)动物/细胞模型,检测MTERFs的蛋白表达变化情况。结果 MTERFs在小鼠脑中和SH-SY5Y细胞中蛋白表达水平较高;MPTP/MPP+诱导的PD模型中,MTERF3蛋白表达水平明显降低。结论在小鼠脑和SH-SY5Y细胞中,MTERFs表达较高;MPTP/MPP+诱导下MTERF3蛋白表达明显降低,影响了线粒体转录水平,这可能参与了帕金森病发生的过程。Objective To investigate the expression of mitochondrial transcription termination factors（MTERFs）in various tissues and cells,and the changes of MTERFs induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）/1-methyl-4-pheny lpyridine（MPP＋）.Methods Western blotting was used to measure the expression of the MTERFs in brain,liver,kidney of C57 BL mice and in human SH-SY5 Y,HCM,L-02 cells.MPTP/MPP＋was injected to imitate PD animal/cell models.The changes of MTERFs protein were detected through western blotting.Results The expression of MTERFs was higher in mice brain and SH-SY5 Y cells.In PD mouse model induced by MPTP,MTERF3 reduced significantly.Conclusion The expression of MTERFs was higher in mouse brain and SH-SY5 Y cells.After induced by MPTP/MPP＋,MTERF3 decreased obviously,which increased transcription level and might involve in a process of Parkinson’s disease.

关 键 词：线粒体转录终止因子 1-甲基-4-苯基-1 2 3 6-四氢吡啶/1-甲基-4-苯基吡啶 帕金森病 免疫印迹法 小鼠 

分 类 号：R394.2[医药卫生—医学遗传学]