基于数学模型的高危HPV E6/E7 mRNA定量分析在宫颈病变筛查中的作用  

Application of mathematic model-based quantitative analysis of high-risk human papilloma virus E6/E7 mRNA in cervical lesion screening

作　　者：徐瑛[1] 高志斌[1] 黄丽丽[1] 熊雅波[1] 赵莺[1] XU Ying GAO Zhibin HUANG Lili XIONG Yabo ZHAO Ying(Department of Pathology, Yuyao People＇s Hospital, Yuyao 315400, China)

机构地区：[1]余姚市人民医院病理科,浙江余姚315400

出　　处：《中国医学物理学杂志》2017年第1期76-79,共4页Chinese Journal of Medical Physics

基　　金：余姚市级科技计划项目(2014Y06)

摘　　要：目的:研究高危人乳头瘤病毒(HPV)E6/E7 mRNA定量分析在宫颈病变筛查中的作用。方法:采用原位杂交技术对440例不同宫颈病变[炎症、低级别上皮内瘤变(CIN)、高级别CIN、浸润性鳞癌]患者行高危HPV E6/E7 mRNA拷贝数进行定量检测,建立数学模型对检测结果进行分析。结果:4组之间HPV E6/E7 mRNA阳性率不全相等,具有统计学差异(P<0.005 0);炎症组与其他3组宫颈病变之间高危HPV E6/E7 mRNA阳性率均有统计学差异(P<0.008 3)。拷贝数大小与病变程度呈正相关,可知当mRNA小于500时,炎症的可能性比较高;500~10 000时,很可能是CIN(包括低级别和高级别);大于10 000时,很可能是高级别CIN和癌症。结论:高危HPV E6/E7 mRNA拷贝数大小与宫颈病变程度正相关,其定量检测可作为宫颈病变联合筛查的一种有效补充工具。Objective To study the value of quantitative analysis of high-risk human papilloma virus（HPV） E6/E7 mRNA in cervical lesion screening. Methods The copies of high- risk HPV E6/E7 mRNA were quantitatively detected using in- situ hybridization technique in 440 patients with different cervical lesions, including inflammatory lesions, low- grade cervical intraepithelial neoplasia（CIN）, high-grade CIN, squamous-cell carcinoma. A mathematic model was used to analyze the results of the tests. Results The positivity rates of high-risk HPV E6/E7 mRNA differed significantly among the 4 groups（P〈0.005）.The positivity rates of high-risk HPV E6/E7 mRNA showed significant differences between the inflammatory lesions and the other 3 lesions（P〈0.008）. The copies of high- risk HPV E6/E7 mRNA were positively correlated with the severity of the lesions, and a mRNA copy number less than 500 suggested a high probability of inflammatory lesions, whereas a copy number of 500- 10 000 indicated a likely diagnosis of CIN（including low- and high- grade ones）; a mRNA copy number exceeding10 000 may support the diagnosis of high-grade CIN or cancer. Conclusion The copy number of high-risk HPV E6/E7 mRNA is positively correlated with the pathological grade of the cervical lesion. Quantitative detection of high- risk HPV E6/E7 mRNA can serve as a complementary examination for cervical lesion screening.

关 键 词：人乳头瘤病毒 宫颈病变 拷贝数 原位杂交技术 数学模型 

分 类 号：R365[医药卫生—病理学]