结冷胶与聚乙二醇丙烯酸酯双网络凝胶的制备及生物相容性评价  

Preparation and Properties of Double Network Hydrogels Based on Gellan Gum and Polyethylene Glycol Acrylate

作　　者：汪争光 胡朵 吴东蔚 鲁路[1] 周长忍[1] WANG Zhengguang HU Duo WU Dongwei LU Lu ZHOU Changren(Department of Materials Science and Engineering, Jinan University, Guangzhou 510630, China)

机构地区：[1]暨南大学材料科学与工程系,广州510632

出　　处：《高等学校化学学报》2017年第2期275-283,共9页Chemical Journal of Chinese Universities

基　　金：广东省自然科学基金(批准号:2014A030313379);国家自然科学基金(批准号:81171459)资助~~

摘　　要：针对结冷胶脆性较大的问题,将聚乙二醇丙烯酸酯(PEGDA)引入结冷胶,通过紫外交联制备了结冷胶/PEGDA双网络凝胶,并对单组分凝胶和双网络凝胶的溶胀性能、微观形貌、拉伸力学性能、动态压缩性能和流变性能等进行比较.结果表明,双网络凝胶在类生理环境中具有较小的溶胀率和较好的尺寸稳定性,PEGDA的引入能够大幅度提高结冷胶的韧性,双网络凝胶的拉断伸长率可达340%,断裂能达1.01×103J/m2,与天然关节软骨相当.将成纤维细胞种植在凝胶内部进行体外三维立体培养,结果显示,细胞在凝胶内部生存状态良好,双网络凝胶的细胞负载率高于单网络结冷胶,说明该体系在生物医用领域具有良好的应用前景.Hydrogels have the similar 3D micro-environment as the native extracellular matrix and hold great potential in future biomedical applications. However the mechanical performance limited their applications. The double network hydrogel（ DN gel） attracts much attention due to its high mechanical properties. This study aimed to prepare a series of DN gels based on gellan gum and polyethylene glycol acrylate（ PEGDA） to improve the toughness of gellan gum hydrogel for 3D cell culture and tissue repair. The DN hydrogels exhibited better mechanical properties,with the tensile fracture energy up to 1. 01 × 10^3 J / m^2,which was similar to the value of natural articular cartilage. Our study suggests that the DN gel based on gellan gum and PEGDA present promising mechanical performance and cell seeding efficiency. These hydrogels may have prospective applications in the fields of wound dressing,artificial cartilage and tissue engineering scaffold materials which require high mechanical properties.

关 键 词：结冷胶 双网络凝胶 聚乙二醇丙烯酸酯 细胞三维立体培养 

分 类 号：O631[理学—高分子化学] TB324[理学—化学]