乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义  

Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue

作　　者：吴旖琦[1] 刘颖[2] 赵丽 金晓明[1] 

机构地区：[1]哈尔滨医科大学基础医学院病理教研室,哈尔滨150081 [2]黑龙江省医院烧伤科 [3]哈尔滨市儿童医院重症医学科

出　　处：《实用肿瘤学杂志》2017年第1期7-12,共6页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究面上项目(12541361)

摘　　要：目的研究乙型肝炎病毒(Hepatitis B virus,HBV)感染与胆管癌(Cholangiocarcinoma,CC)的关系,并明确其与肝内胆管癌(Intrahepatic cholangiocarcinoma,ICC)和肝外胆管癌(Extrahepatic eholangiocarcinoma,ECC)发生的关系。方法采用免疫组化、巢式PCR对52例CC(21例肝内胆管癌和31例肝外胆管癌)石蜡包埋组织进行HBV相关蛋白及基因的检测。结果 21例ICC组织中HBsAg阳性表达率为23.8%(5/21),HBcAg阳性表达率为19.0%(4/21),HBV X基因检出率为33.3%(7/21)。在31例ECC组织中均未检出HBsAg、HBcAg及HBV X基因。结论 HBV感染与ICC发生关系密切,但可能非ECC发生的危险因素。HBV X基因的整合可能参与肝内胆管上皮细胞的癌变过程。Objective To investigate the importance of hepatitis B virus （ HBV） infection in the patho-genesis of cholangiocarcinoma（CC） and further clarify the correlation between the occurrence of intrahepatic and extrahepatic cholangiocarcinoma. Methods HBV protein and gene of 52 formalin fixed,paraffin embedded tis-sues with CC were detected by immunohistochemistry and nest PCR. Results Hepatitis B virus X gene was de-tectable in 33. 3% （7/21）of 21 intrahepatic cholangiocarcinoma cases. Hepatitis B surface antigen（ HBsAg） was detectable in 21.7% （5/21 ）and hepatitis B core antigen（ HBcAg）was detectable in 19.0% （4/21 ）of 21 intrahe-patic cholangiocarcinoma cases. In contrast,no HBsAg,HBcAg and hepatitis B virus X gene were detected in any of the 31 extrahepatic cholangiocarcinoma cases. Conclusion HBV infection is a significant risk factor for intra-hepatic cholangiocarcinoma,but not for extrahepatic cholangiocarcinoma,in Northeast of China. The integration of HBV gene may be involved in the carcinogenesis of intrahepatic bile duct epithelial cells.

关 键 词：胆管癌 乙型肝炎病毒 肝内胆管癌 肝外胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]