两步酶解提取米糠蛋白的工艺研究及优化  

Study and Optimization on the Extraction of Rice Bran Protein by Two-Step Enzymatic Hydrolysis

作　　者：王乔[1] 侯同刚[1] 咸漠[1] 王兰[1] 陈盼盼[1] 王建勋[1] WANG Qiao HOU Tonggang XIAN Mo WANG Lan CHEN Panpan WANG Jianxun(Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China)

机构地区：[1]中国科学院青岛生物能源与过程研究所中国科学院生物基材料重点实验室,山东青岛266101

出　　处：《氨基酸和生物资源》2016年第4期77-83,共7页Amino Acids & Biotic Resources

摘　　要：以去除淀粉后的米糠残渣为原料,对复合酶法提取米糠蛋白工艺进行了研究及优化,以期提高米糠蛋白的产品得率和品质。首先利用复合纤维素酶破坏米糠细胞壁结构,充分释放并暴露米糠蛋白,其较优条件为:复合纤维素酶用量为0.2%、温度为60℃、pH=6.0、醪浓度为12%。在此基础上,利用碱性蛋白酶进一步水解米糠蛋白,并在单因素试验的基础上,通过响应面法进一步优化得到最优的水解条件为:温度88℃、pH=8.5、酶用量2.11%。通过上述两步酶解工艺,米糠蛋白的提取率为85.3%。Extraction of rice bran protein by two-step enzymatic hydrolysis was studied and optimized in order to enhance the product yield and quality by using the rice bran residue without starch. First, the composite cellulase was used to destruct the cell wall structure of rice bran, achieving the goal to fully release and expose the rice bran protein. The optimum conditions were obtained as follows： 0.2% of enzyme dosage, 60℃, pH 6.0, 12% of mash concentration. Furthermore, the released and exposed rice bran protein was enzymatically hydrolyzed by alkaline protease. On the basis of single factor experiment, the process was further optimized by response surface methodology and the optimal process conditions were obtained as follows： 2.11% of enzyme dosage, 88℃, pH 8.5. Finally, the extraction rate of rice bran protein was 85.3%.

关 键 词：米糠蛋白 两步酶解 复合纤维素酶 碱性蛋白酶 响应面法 

分 类 号：Q512[生物学—生物化学]